烟夜蛾性信息素结合蛋白2(PBP2)基因的克隆、序列分析与时空表达  

Cloning,sequence analysis and spatio-temporal expression of a pheromone binding protein 2 (PBP2) gene from Helicoverpa assulta (Guenée) (Lepidoptera:Noctuidae)

作　　者：李亮[1] 杨文玲[1] 郭线茹[1] 罗梅浩[1] 原国辉[1] 乔奇[1] 付晓伟[1] 

机构地区：[1]河南农业大学植物保护学院,郑州450002

出　　处：《昆虫学报》2009年第11期1199-1205,共7页Acta Entomologica Sinica

基　　金：河南省杰出青年科学基金项目(074100510013)

摘　　要：性信息素结合蛋白(pheromone binding proteins,PBPs)在昆虫雌雄间信息交流中起着重要作用。本研究利用RT-PCR和RACE方法,从烟夜蛾Helicoverpa assulta(Guenée)雄虫触角中克隆了性信息素结合蛋白2基因的开放阅读框及3′末端序列,该基因被命名为HassPBP2(GenBank登录号为EU316186)。克隆和测序结果表明,HassPBP2开放阅读框全长450bp,编码149个氨基酸残基,推测编码蛋白的分子量为16.9kD,等电点为5.56。HassPBP2基因结构分析表明,该基因由3个外显子和2个内含子组成,内含子的长度分别为90和261bp。氨基酸序列联配分析表明,此序列具有气味结合蛋白的典型特征,与其他鳞翅目昆虫PBPs的一致性在34%~91%之间,其中与棉铃虫Helicoverpa armigeraPBP2和烟芽夜蛾Heliothis virescensPBP2的序列一致性高达91%。时间表达和组织表达分析显示,HassPBP2在卵期、幼虫期和蛹早期不表达,在蛹中期开始表达,并一直持续到成虫中期,且只在雌、雄成虫触角中表达。Pheromone binding proteins(PBPs)play an important role in communication between male and female insects.The open reading frame and 3' end full-length cDNA of a novel pheromone binding protein 2 gene was cloned from Helicoverpa assulta(Guene)by using RT-PCR and RACE methods,which was named HassPBP2(GenBank accession no.EU316186).The cloning and sequencing results showed that the full-length of open reading frame in HassPBP2 was 450 bp,encoding 149 amino acid residues with the predicted molecular weight and ...

关 键 词：烟夜蛾 性信息素结合蛋白 基因克隆 序列分析 时空表达 

分 类 号：S433[农业科学—农业昆虫与害虫防治]