聚天冬氨酸对庆大霉素抑制耳蜗肌醇脂质信使系统的影响  

Effect of polyaspartic acid on gentamicin-inhibiting inositidelipid messenger system in guinea pig cochlea

作　　者：郭玉芬[1] 姜泗长[2] 杨伟炎[2] 韩东一[2] 

机构地区：[1]兰州医学院第二附属医院耳鼻咽喉科,兰州730030 [2]中国人民解放军总医院耳鼻咽喉-头颈外科,北京100853

出　　处：《中华耳科学杂志》2003年第3期20-23,共4页Chinese Journal of Otology

摘　　要：目的观察聚天冬氨酸(polyaspartic acid,PAA)对庆大霉素(gentamicin,GM)抑制耳蜗肌醇脂质信使系统的影响,进一步探讨PAA对庆大霉素耳毒性拮抗作用机制.方法采用[3H]-肌醇参入法和高效液相色谱分析法(high performance liquid chromatography,HPLC)测定不同给药组、不同给药时间豚鼠耳蜗组织肌醇磷酸酯(inositolphosphates,IPs)、多磷酸肌醇磷脂(polyphosphatidylinositol,PPI)及磷脂酶C(PPI phospholipase C,PLC).结果①给药1天后不同给药组间耳蜗组织IPs、PPI及PLC均无显著性差异(P＞0.05).②给药5天后单用GM组耳蜗组织PPI增加、Ips和PLC减低,与PAA+GM组及其他二对照组间有显著性差异(P＜0.01).③给药10天后单用GM组耳蜗组织PPI、IPs及PLC与PAA+GM组及其他二对照组间有显著性差异(P＜0.01),但与单用GM5天相比,PPI变化不明显,IPs和PLC升高.结论PAA对GM抑制耳蜗肌醇磷酸酯信使系统有拮抗作用,从而起到对GM耳毒性连锁反应早期阶段的保护作用.Objective To observe the efiect of polyaspartic acid (PAA) on gentamicin - inhibiting inositidelipid messenger system in guinea pig cochlea and investigate the protective mechanism of polyaspartic acid on gentamicin - ototoxicity. Methods By using incorporation of [3H] - inositol and high performance liquid chromatography (HPLC) technique to determine inositol phosphates (IPs), polyphosphatidylinositol (PPI) and PPI phospholipase C (PLC) in guinea pig cochlea at different using drug time and in different using drug groups. Results①After using drug 1 - day, there was not significant difference of IPs, PPI and PLC among four groups (P > 0.05 ) . ② After using drug 5 ?days , compared with PAA + GM group and other two control groups, there was a significant difference of IPs,PPI and PLC in using only GM group (P< 0.01) , PPI was increase, IPs and PLC were decrease.③After using drug 10- days , compared with PAA+ GM group and other two control groups, there was a significant difference of Ips, PPI and PLC in using only GM group(P< 0.01) ; but, compared with using only GM 5-days, there was not sinigficant change of PPI, IPs and PLC were increase. Conclusion PAA has significance preventing effects on gentamicin - inhibiting inositidelipid messenger system in guinea pig cochlea , which shows that PAA has protective effect on gentamicin - ototoxicity in guinea pig.

关 键 词：聚天冬氨酸 庆大霉素 第二信使系统 耳蜗 [3H]-肌醇 HPLC 

分 类 号：R76[医药卫生—耳鼻咽喉科]