白细胞介素2提高HBV基因疫苗诱导的体液免疫应答  

Interleukin-2 promotes the specific humoral immune response induced by hepatitis B viral gene vacdne

作　　者：杜德伟[1] 白宪光[1] 冯志华 姚志强[1] 周永兴[1] 

机构地区：[1]西安市第四军医大学唐都医院全军感染病诊疗中心,710038

出　　处：《实用肝脏病杂志》2003年第2期78-79,共2页Journal of Practical Hepatology

基　　金：国家自然科学基金资助项目(39770665)

摘　　要：目的观察IL-2真核表达载体对HBV基因疫苗诱导BALB/c小鼠的特异性体液免疫应答的影响。方法肌肉注射基因疫苗及IL-2真核表达载体,ELISA法检测小鼠血清抗-HBs。结果免疫8周后,单纯注射pCR3.1-S及共注射IL-2真核表达载体的小鼠血清450nm A值分别为1.24±0.10及1.98±0.17,两组相比有明显差异(P<0.01)。结论IL-2的真核表达载体能够提高小鼠对基因疫苗的体液免疫应答。Objective To observe the effect of interleukin-2 eukaryotic expression plasmid on specific humoral immune response to gene vaccines coding HBV surface antigen in Balb/c (H - 2d) mice. Methods The immunization was performed by intramuscular injection in this experiment. Anti-HBs in serum was detected by ELISA. Results 8wks after immunization, the value of 450nm of mouse sere codelivered with IL-2 vaccine (1.98 ± 0.17)was significantly higher than that of mice injected HBV - S gene vaccine alone (1.24 ±0.10). Conclusion The results showed that the HBV gene vaccine( pCR3.1 - S) had strong antigenecity which can be promoted by vector coding murine IL-2.

关 键 词：基因疫苗 乙型肝炎表面抗原 IL-2 小鼠 

分 类 号：R392[医药卫生—免疫学]