复方丹参对Ⅱ型胶原蛋白诱导大鼠模型滑膜细胞分泌及肿瘤坏死因子的影响  

Effect of salvial miltiorrhizal(sm) on PGE2 and TNF secretion by synovlocytes in CIA rats

作　　者：黄清春[1] 张声鹏[2] 徐秋英[2] 陈光星[2] 沈鹰[1] 黄伟毅[1] 马伟[2] 

机构地区：[1]解放军广州军区广州总医院,广东广州510010 [2]广州中医药大学,广东广州510120

出　　处：《中国组织工程研究与临床康复》2001年第19期54-55,共2页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省科委重点攻关项目99M044087G

摘　　要：目的研究复方丹参Ⅱ型胶原蛋白诱导性大鼠关节模型CIA关节功能及滑膜细胞分泌前列腺素E2PGE2及肿瘤坏死因子TNF的影响。方法采用消化分离的方法获得滑膜细胞,放免法检测原代大鼠滑膜细胞培养上清液中PGE2及TNF的水平。结果复方丹参可以明显下调CIA大鼠滑膜细胞分泌PGE2及TNF的水平,改善其关节活动功能。结论复方丹参治疗类风湿性关节炎的机制可能与下调滑膜细胞分泌PGE2及TNF的水平有关。Objective To srudy the effect of salvial miltiorrhizal(sm) on the PGE2 and TNF secretion by synovlocytes in CIA rats.Method Synovlum of knee joints from CIA rats were digested by collagenase and trlosln to prepare DSSC(digested synovlal single cells).The content of the supernatant fluid were assayed by the radlolmmunoassay.Result SM could down-regulated PGE2 and TNF productions of DSSC.Conclusion The mechanism of curative effct of salvial miltiorrhizal on arthritis concern with depressing PGE2 and TNF productions of DSSC.

关 键 词：类风湿性关节炎 滑膜细胞培养 肿瘤坏死因子 前列腺素 复方丹参注射液 

分 类 号：R593.22[医药卫生—内科学]