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作 者:吕路[1] 黄锋先[2] 张增华[1] 桓文穆[1] 黄华[1]
机构地区:[1]广州铁路中心医院肾内科,广州510080 [2]中山大学附属第一医院肾内科,广州510089
出 处:《中国血液净化》2002年第7期3-6,共4页Chinese Journal of Blood Purification
基 金:广东省科委自然基金(No.960615);广州铁路集团公司科研开发项目(No.2001-3.26)基金
摘 要:目的 了解不同透析膜对血液透析患者IL-8表达的影响。方法 采用酶联免疫吸附试验(ELISA)测定IL-8血浆水平及外周血单个核细胞(PBMC)培养上清液IL-8水平,逆转录多聚酶链反应(RT-PCR)检测基因表达。结果 聚砜膜组的IL-8血浆水平及mRNA表达低于铜仿膜组及铜氨膜组,而铜仿膜组及铜氨膜组之间无显著差异。各组透析后的IL-8血浆水平及mRNA表达均较透析前升高。铜仿膜组透析前PBMC受LPS刺激后产生的IL-8多于聚砜膜组,血透后聚砜膜组产生的IL-8较透析前升高,而铜仿膜组较透析前无明显改变。结论 再生纤维素膜(尤其是铜仿膜)导致IL-8表达改变的能力强于聚砜膜,测量IL-8表达有助于评价不同透析膜的生物相容性。Objective To investigate the influence of the different dialysis membrane on the interleukin - 8 expression in uremic patients. Methods The plasma concentration of IL- 8 and the IL- 8 production by the cultivated PBMC were measured with enzyme - linked immunoabsorbent assay (FLISA). The gene expression of IL-8 was measured with reverse transcription - polymerase chain reaction(RT - PCR) . Results The IL - 8 plasma and mRNA level of the patients using the regenerated cellulose membrane (cuprophan CU, cuprammonium rayon CR) is higher than that of those using polysulfone (PS) membrane. The IL- 8 plasma level and mRNA expression increases after hemodialysis. The production of IL- 8 from PBMC of the patients using CU membrane was more than that of those using PS membrane. In PS group the IL - 8 production of PBMC increased after dialysis. But there was no obvious difference of IL - 8 production from PBMC between pre - dialysis and post - dialysis in CU group. Conclusion The change of IL - 8 mRNA expression and plasma level through the hemodialysis in the regenerated cellulose membrane (especially CU) groups is more significant than that in PS group. The measurement of IL - 8 expression is useful to evaluate the biocompatibility of dialysis membranes.
关 键 词:血液透析 白细胞介素8(IL-8) 透析膜 尿毒症
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