雄激素对老龄大鼠血管平滑肌组织雄、雌激素受体基因表达的影响  

The effect of androgen on androgen receptor and estrogen receptor-β expression in vascular smooth muscle of old rats

作　　者：吴赛珠[1] 吕红松[1] 吴迎星[1] 马瑞[1] 王子东[1] 郑华[1] 杨春莉[1] 赖文岩[1] 

机构地区：[1]解放军第一军医大学南方医院心血管内科,广州510515

出　　处：《中华老年多器官疾病杂志》2002年第2期114-116,共3页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金："973"国家重点基础研究发展规划基金资助项目(G2 0 0 0 5 70 0 8) ;国家自然科学基金 (3 960 0 169) ;军队医药卫生科研基金重点项目 (0 1Z0 42 )

摘　　要：目的　探讨不同剂量的庚酸睾酮 (TE)对老龄雄性大鼠血管平滑肌组织中雄激素受体 (AR)及雌激素受体 β(ER β)基因表达的影响。方法　 2 1月龄的老龄雄性Sprague Dawley纯系大鼠 4 0只随机分为A组 (老龄对照组 )、B组 (老龄低剂量组 ,肌注每次TE 2 .5mg/kg ,1次 /周× 16周 )、C组 (老龄中剂量组 ,肌注每次TE 5 .0mg/kg ,1次 /周× 16周 )、D组 (老龄高剂量组 ,肌注每次TE 10 .0mg/kg ,1次 /周× 16周 )。Western印迹法检测血管平滑肌组织中AR及ER β基因表达情况。结果　给予低、中、高 3种剂量的TE作用后血管平滑肌组织AR蛋白条带吸光度分别为 (99.5 0± 2 1.74 ) ,(12 5 .38± 2 8.6 8) ,(10 1.98± 15 .4 2 ) (对照组为 10 0 ) ;ER β蛋白条带吸光度分别为(92 .34± 18.6 8) ,(47.72± 18.12 ) ,(82 .13± 2 3.5 0 ) (对照组为 10 0 )。结论　中剂量的TE可促进AR基因的表达量 ,而中、高剂量的TE则抑制ER β基因的表达量。Objective To investigate the effects of testosterone enanthate (TE) on androgen receptor (AR) and estrogen receptor β( ER β) expression in vascular smooth muscle of old rats. Methods Fourty aged male rats were randomly divided into 4 groups:group A(placebo group),group B (intramuscular injection of TE 2.5mg/kg , once a week),group C(intramuscular injection of TE 5.0mg/kg , once a week),group D (intramuscular injection of TE 10.0mg/kg , once a week).All animals were fed freely during the 16 week treatment period.The expression of AR and ER β was studied with Western blotting.Results AR expression in vascular smooth muscle tissue was regulated by TE,with protein band intensity being( 99.50±21.74),(125.38±28.68) and (101.98±15.42) for TE doses of 2.5,5.0 and 10.0 mg/kg, respectively.The expression of ER β was also regulated by TE,with protein band intensity being(92.34± 18.68 ),(47.72±18.12),(82.13±23.50) (the data were expressed as ±s,with control group taken as 100)for these groups,respectively.Conclusions TE at mid dose (5.0mg/kg,once a week) stimulates the expression of AR,whereas the mid and high doses (5.0 and 10.0 mg/kg,once a week) inhibit the expression of ER β.

关 键 词：睾酮 受体 雄激素 受体 雌激素 大鼠 

分 类 号：R5[医药卫生—内科学]