糖皮质激素对骨髓基质细胞增殖及分化的影响  

The effect of glucocorticords on bone marrow stromal cell proliferation and differentiation

作　　者：李月白[1] 秦国斌[2] 殷力[3] 许建中[3] 王义生[3] 

机构地区：[1]河南医科大学基础医学院生化教研室 [2]安阳市第一人民医院骨科 [3]河南医科大学第一附属医院骨科,郑州450052

出　　处：《中国骨肿瘤骨病》2002年第3期169-171,共3页Chinse Journal Of Bone Tumor And Bone Disease

基　　金：河南省科委自然科学基金资助项目(编号:984021700)

摘　　要：目的 观察糖皮质激素对骨髓基质细胞增殖及分化的作用,探讨糖皮质激素导致继发性骨质疏松的病理学机制。方法 以1×10-7M地塞米松加入骨髓基质细胞培养物中,测定增殖的骨髓基质细胞数及培养液中骨钙素含量。通过苏丹Ⅲ脂肪细胞染色计数观察地塞米松作用时间对脂肪细胞分化的影响。结果 实验组骨髓基质细胞数及培养液中骨钙素含量明显低于对照组,脂肪细胞的数量随地塞米松作用时间延长而增多。结论 糖皮质激素抑制骨髓基质细胞增殖及向成骨细胞分化,促进其向脂肪细胞分化,这可能与糖皮质激素引起继发性骨质疏松时骨量减少、髓内脂肪组织增多有关。Objective To observe the effect of glucocorticoids on bone marrow stromal cell proliferation and differntiation and study the pathogenetic mechanism of osteoporosis. Methods Dexamethasone (1×10-7 M) was added to the cell culture medium of bone marrow stromal cells, cell count was done on the proliferated bone marrow cells and the contents of osteocalcin in the media was measured. Results The number of the bone marrow stromal cell and the contents of osteocalcin in the media of the experimental groups obviously decreased compared with the control. The number of adipocytes increased with longer duration of exposure to Dexamethasone. Conclusion Glucocorticoids inhibited bone marrow stromal cell proliferation, and its differentiation into osteoblasts; it promoted the differentiation of the stromal cells into adipocytes. This might be a major contributing factor of bone loss and an increase in marrow fat volume in osteoporosis.

关 键 词：糖皮质激素 骨质疏松 骨髓 基质细胞 

分 类 号：R58[医药卫生—内分泌]