反义IGF-Ⅱ基因对胃癌细胞恶性表型的影响  

Effect of antisense IGF- Ⅱ gene on malignant phenotype of gastric cancer cells

作　　者：张鸣青[1] 吴乔[2] 刘庆春[1] 仇日火[1] 

机构地区：[1]解放军175医院内二科,福建漳州363000 [2]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《福建分析测试》2001年第2期1409-1411,共3页Fujian Analysis & Testing

基　　金：漳州市科委重大科研资助Z9938

摘　　要：研究反义IGF-Ⅱ基因对胃癌细胞恶性表型的影响。将已构建的反义IGF-Ⅱ基因真核表达载体pIGF-ⅡAs导入人胃癌细胞BGC-823经G418抗性筛选,获得转基因细胞株克隆,测定细胞生长曲线,扫描电镜观察细胞表面结构,观察软琼脂细胞集落形成能力。结果转染反义IGF-Ⅱ基因细胞生长受抑制,细胞软琼脂集落形成能力下降。扫描电镜显示:转染反义基因细胞表面微绒毛减少。转染IGF-Ⅱ反义基因抑制胃癌细胞恶性表型.转染反义IGF-Ⅱ基因可能成为潜在的胃癌治疗手段。Inhibitory effect of insulin like growth factor Ⅱ (IGF-Ⅱ) antisense RNA on malignant phenotype of gastric cancer cells was studied. IGF-Ⅱ antisense RNA expression vector of pIGF-Ⅱ As was obtained. The pIGF-Ⅱ As was then introduced into human gastric cancer cell line BGC-823; The resistant to G418 colonies was isolated. The cells growth rate and soft agar colony formation were investigated. The cells entity was observed with scanning electron microscopy. Results The IGF-Ⅱ antisense gene suppressed cell growth curve. There was significantly inhibitions on soft agar colony formation as showed in scanning electron micrograph. The fewer thicker and shorter microvillies are revealed on the surface of IGF-antisense gene transfected cell lines. Conclusion IGF-Ⅱ antisense gene inhibited malignant phenotype of gastric cancer cells.

关 键 词：反义IGF-Ⅱ基因 胃癌细胞 恶性表型 生长抑制 

分 类 号：TB9[一般工业技术—计量学]