冷冻保存对人同种带瓣管道力学特性的影响  

Effect of Cryopreservation on Biomechanical Properties of Human Valved Conduit

作　　者：刘金平[1] 孙宗全[1] 董念国[1] 付平[1] 储丽磊[1] 

机构地区：[1]华中科技大学同济医学院协和医院心外科,武汉430022

出　　处：《华南国防医学杂志》2003年第3期1-3,共3页Military Medical Journal of South China

摘　　要：目的:探讨低温液氮保存对同种带瓣管道的生物力学特性的影响,为临床确定同种带瓣管道的最佳保存时间提供理论依据。材料与方法:取人同种带瓣主动脉24根,其中21根同种带瓣主动脉灭菌后,置入深低温冰箱梯度降温,待降至-80℃后转入-196℃液氮保存,按保存时间不同分为七组(A、B、C、D、E、F、G),每组三份,另外3根新鲜带瓣主动脉设为对照组。复温后将主动脉壁血管片置入生物力学检测仪行拉伸实验,测定其最大负载,最大拉伸及弹性模量,并行组织学检测观察组织结构变化。结果:主动脉血管片力学测试结果见保存12个月内的主动脉壁其最大负载、最大延伸及弹性模量与新鲜组比较无明显差异(P >0.05),而保存12个月后其弹性模量、最大负载、最大延伸均显著下降(P<0.05)。组织学检查可见,保存12个月内的主动脉壁其纤维结构保存完好。结论:低温液氮保存12个月内,同种带瓣管道可保持组织结构完整,并维持其生物力学特性。Objective 16 investigate the biomechanical properties of human valved conduit cryopreserved in liquid nitrogen. Methods Twenty-one human aortic valved conduits, which were obtaind from brain death donors and cryopreserved in liquid nitrogen from 1 month to 3 years, were divided into seven groups(A,B,C,D,E,F,G) according to the preservative time. Each group contained 3 valved conduits, and another 3 fresh valved conduits were serve as the control group. The elastic modulus, maximal stress, and maximal load of the aortic walls were determined to know their dynamic properties. The histological examination was performed to study the change in the histological structure of these coaduits walls after rewarming them after cryopreservation. Results The elastic modulus, maximal load and maximal stress of aortic walls decreased significantly in groups F and G, where the valved conduits were cryopreserved for more than 12 months, as compared with that of the fresh conduits ( P <0.05). The collagen fibrils and elastic fibrils of the aortic walls were intact and arranged orderly within 12 months after the cryoprseervation. Conclusion The structure and biomechanical properties of human valved conduits within 12 months after their cryopreservation in liquid nitrogen can be maintained.

关 键 词：低温保存 同种血管 生物力学 

分 类 号：R322.121[医药卫生—人体解剖和组织胚胎学]