计算机辅助诊断系统对小鼠胸腺细胞凋亡超微结构实验研究  

Ultrastructural Study of Apoptosis of Thymocytes by Computer-assisted Tomography in Mce.

作　　者：欧陕兴[1] 杨静[1] 吴迪[1] 余浩杰[1] 萧湘生[2] 

机构地区：[1]广州军区广州总医院放射科,广州510010 [2]第二军医大学附属长征医院影像科

出　　处：《华南国防医学杂志》2003年第5期6-8,共3页Military Medical Journal of South China

摘　　要：目的:利用fPAX^TM计算机辅助诊断系统评估经糖皮质激素诱导小鼠体外胸腺细胞凋亡超微结构图像。方法:先对小鼠腹腔内注射地塞米松诱导细胞凋亡;利用fPAX^TM计算机辅助诊断系统后处理功能,按照实验程序对扫描与透射电镜图像进行数码采集、观察与评估。结果:按照程序对经fPAX^TM计算机辅助诊断系统后处理的电镜图像在线分析,结果显示在图像质量与图像参数实验组与对照组之间存在显著性差异(P<0.05)。结论:经fPAX^TM计算机辅助诊断系统后处理的电镜图像质量高,清晰地显示细胞凋亡细节包括细胞凋亡不同时期的超微结构。Objective To study the ultrastructural features of apoptosis of thymocytes induced by glucocorticoid in mice. Methods Dexamethasone was intraperitoneally injected to induce apoptosis of thymocytes in the nice. The atrophied thymus was examined by electron microscopy. The pictures in ultrastructure of the thymocytes were processed and analyzed by computer-assisted tomography. Results The characteristics of apoptosis appeared in the thymocytes at different stages in the mice treated by dexamethasone. There are significantly difference in qualiy and some parameters of the ultrastructural pictures between both the treatment and the control groups ( P <0.05). Conclusion Computer assisted tomography is a very useful method for improving quality of imagings to evaluate apoptosis of thymocytes induced by glucocorticoid.

关 键 词：胸腺细胞凋亡 实验研究 计算机X线摄像 鼠 

分 类 号：R392[医药卫生—免疫学]