三元溶液相图的绘制及其在血小板低温保存中的应用  

Phase Diagram for the Ternary System and Its Application to Platelet Cryopreservation

作　　者：刘景汉[1] Dayong Gao 何立群[2] 欧阳锡林[1] 刘作斌[1] 

机构地区：[1]解放军总医院输血科,解放军临床输血中心,北京,100853 [2]中国科技大学热科学与能源工程系低温生物医学研究所

出　　处：《华南国防医学杂志》2003年第6期1-3,共3页Military Medical Journal of South China

基　　金：国家自然基金项目(79930812)

摘　　要：目的:获得血小板冰冻保护液(丙二醇/氯化钠/水,PG/NaCl/H2O)冰冻降温的数学模型。方法:使用差示扫描量热仪分别测定出不同浓度和不同比例条件下丙二醇/氯化钠/水三相溶液的凝固点(Tm),然后将已有的公式和实验所得数据通过计算机进行拟合,获得—个数学模型。结果:该数学模型描述的是在PG/NaCL/H2O溶液中Tm变化与溶质浓度和PG/NaCl(质量比)两个参数变化之间的函数关系。结论:研究提示把该方程与血小板的渗透性特性的参数相结合,可以描述在平衡冷冻状态下,不同PG浓度时血小板的体积变化,指导血小板低温保存。ObjectiveTo acquire freezing model of the cryopretective solution (H2O- NaCL- propyleneglycol ternary systen) for platelets. Methods The melting points(Tm) of the cryopreservation solutions which were composed of H2O, Nad and propyleneglycol at the different rate among them, were measured by using a differential scanning calorimeter (DSC). These experimental data were treated by a computer. Result An empirical equation which characterized the Tm as a function of the solute concentration and the ratio of propyleneglycol mass to NaCl mass inside this solution, was obtained. Conclution The equilibrium freezing model for human platelets in this system was described.

关 键 词：血小板低温保存 低温保护液 相图 熔点 丙二醇 

分 类 号：Q273[生物学—细胞生物学]