枯草芽孢杆菌TH_2菌株抗菌物质产生的技术研究  

STUDY ON FERMENTATION CONDITIONS FOR THE ANTI—FUNGAL—BACTERIUM SUBSTANCE OF THE ANTIBIOTIC—PRODUCING BACTERIUM STRAIN TH_2(BACILLUS SUBTILIS)

作　　者：林国宪 黄勤清 董德臻 高日霞[2] 

机构地区：[1]福建泰禾生物工程研究院,福建福州350012 [2]福建农林大学,福建福州350002

出　　处：《武夷科学》2003年第1期23-28,共6页Wuyi Science Journal

摘　　要：通过正交试验,结果表明枯草芽孢生防菌TH2高产抗菌物质培养基的最佳配方为豆饼粉1.5％,玉米粉为0.5％,麦麸0.5％,大米粉0.5％,最佳的培养条件为通气量25ml装瓶量、pH为7.5、温度为28±1℃,这样产生的抗菌物质最多。枯草芽孢生防菌株TH_2培养过程中,pH从培养初始的7.0,经下降又逐步上升到48小时的7.8。OD650从培养初始的0.56,逐步上升到36小时的1.10,而后逐渐下降,84小时降到0.85。孢子生成从12小时开始,孢子数达(108cfu/ml),而后逐渐增加,48小时达到高峰,孢子数达58.2(108cfu/ml),随后孢子数逐渐下降,抑菌圈值随着发酵时间增加逐渐增大。The present paper dealt with selection of fermentation conditions for the growth of the antibiotic—producing bacterium strain TH_2 (Bacillus subtilis). The results showed that the optimal cultural media consisting of 1.5% bean cake powder, 0.5% corn flour, 0. 5% peptone, according the fermentation test, on which the optimal conditions for the fermentation of strain TH_2 were pH 7.5 and temperature28±1℃. Like this, anti—fungal—bacterium substance is at most. During the fermentation, pH gradually decreased down from 7.0 at the beginning then increased up to 7.8 at 48 hours. OD_(650) raised up from 0.56 at the beginning to 1.10 at 36 hours then decreased down to 0.85 at 84 hours. The peak value of spore generation was 58.2 (10~8cfu/ml) at 48 hours. Inhibition zone along with ferment the time gain to gradually enlarge.

关 键 词：枯草芽孢杆菌(Bacillus subtilis)TH2 培养基配比 培养条件 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]