二次回归旋转组合设计在苏云金杆菌发酵培养基优选中的应用  

OPTIMIZATION OF GROWTH MEDIUM FOR BACILLUS THURINGIENSIS USING QUADRATIC REGRESS ION ROTATIONAL COMBINATIONAL DESIGN

作　　者：陈聪[1] 李今煜[1] 关雄[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室

出　　处：《武夷科学》2005年第1期7-12,共6页Wuyi Science Journal

基　　金：国家高技术发展计划863(2002AA245011);福建省重大科技项目(2002N004)

摘　　要：采用二次回归旋转组合设计方法,对苏云金杆菌WB7菌株的培养基配方进行了摇瓶优化筛选。建立了产孢量与五种原料的数学模型,分析了各试验因子的单因素效应和互作效应。试验所得的最佳培养基组合为:玉米淀粉3.3%,黄豆饼粉4%,酵母粉0.725%,蛋白胨0.4%,鱼粉1.05%。The quadratic regression rotational combinational design was used to screen fermentative medium of Bacillus thuringiensis isolate WB7.The regression equation was established which expresses the number of spores of Bt as function,and the five materials as variable respectively.Using this regression equation, the single factor effect and mutual effect was studied.The most appropriate fermentative media was composed of corn meal 3.3%,soybean flour 4%,yeast powder 0.725%,peptone 0.4% and fish meal 1.05%.

关 键 词：二次回归旋转组合设计 苏云金杆菌 培养基优化 

分 类 号：Q815[生物学—生物工程]