虎杖苷对内毒素刺激下多形核白细胞纤维状肌动蛋白的影响  

Effect of polydatin on F-actin of lipopolysaccharide-incubated Polymorphonuclear leukocytes

作　　者：黄海潇[1] 赵清[1] 金春华[1] 

机构地区：[1]第一军医大学病理生理教研室,广州510515

出　　处：《中国药物应用与监测》2004年第1期21-24,共4页Chinese Journal of Drug Application and Monitoring

基　　金：国家自然科学基金(9870936)

摘　　要：目的:观察虎杖苷对内毒素刺激下多形核白细胞(Polymorphonuclear leukocytes,PMN)骨架蛋白变化的影响,探讨虎杖苷(Polydatin,PD)治疗炎症、感染性休克的机制。方法:以流式细胞仪及共聚焦显微镜观察虎杖苷对内毒素刺激下罗丹明-鬼笔环肽(Rhodamine-Rhalloidin)标记纤维状肌动蛋白(F-actin)的变化。结果:在LPS刺激作用下,PMN F-actin含量明显下降至正常时的80±0.02%(P<0.05),在LPS刺激后再用PD治疗,其F-actin又上升至正常的89±0.02%(P<0.05)。在正常情况下,PMN中F-actin主要分布于细胞膜附近,形成一层较为致密的骨架,支撑起细胞的形状。在LPS刺激后,F-actin在细胞边缘形成一宽厚的骨架层,同时发现该层分布不均匀,提示在一些部位可能会有伪足形成。LPS刺激后再用PD治疗,发现细胞边缘的F-actin较LPS刺激时明显减少,而中央相对增加。结论:PD可能通过抑制LPS对细胞骨架蛋白F-actin的影响发挥抗炎症性休克的作用。Objective:To understand the action mechanisms of polydatin (PD) in the treatment of septic shock in view of its effect on lipopolysaccharide (LPS) -induced F-actin's level and distribution of the polymorphonuclear leukocytes(PMN).Methods:Flow cytometry and confocal microscope were used to investigate the regulative role of PD in Rhodamine-Rhalloidin fabled F-actin of PMN in response to LPS stimulation.Results:Under LPS stimulation, the level of PMN F-actin obviously reduces to 80 ± 0.02% (p<0.05) of the normal. While after PD therapy, its level increases to 89 ±0.018% (p<0.05). In normal situation, inside PMN, F-actin mainly locates nearby cell membrane, forming a quite dense layer of framework to hold the morphology of the cell. After LPS stimulation, F-actin forms a denser skeleton layer at the edge of cell and an uneven distribution. Adding PD treatment after LPS stimulation, F-actin at cell edge becomes much less, while increases in middle obviously.Condusions:PD can inhibit LPS's effect on F-actin, and may play a crucial role in the treatment of inflammation and septic shock.

关 键 词：虎杖苷 多形核白细胞 内毒素 纤维状肌动蛋白 

分 类 号：R285[医药卫生—中药学]