环氧化酶-2抑制剂与抗癌药对Caco-2细胞抑制的相互作用  

Interaction of inhibiting effects of cyclooxygenase-2 inhibitors and anticancer agents on Caco-2 cell strain

作　　者：熊宇亮[1] 龙启才[2] 

机构地区：[1]中山大学药学院,广州510089 [2]中山大学药学院临床药理研究所,广州510089

出　　处：《中国药物应用与监测》2004年第4期55-57,共3页Chinese Journal of Drug Application and Monitoring

摘　　要：目的:探讨环氧化酶-2(COX-2)抑制剂与细胞毒抗癌药对人结肠腺癌细胞Caco-2抑制的相互作用。方法:用MTT法测定尼美舒利和塞来昔布与细胞周期非特异性药物CDDP和细胞周期特异性药物5-Fu并用时Caco-2细胞的抑制作用。结果:塞来昔布1mg/L～10mgL与CDDP 1mg/L合用或塞来昔布1mg/L～5mg/L与5-Fu 0.5mg/L合用,对Caco-2细胞的抑制有相加作用。更高浓度(15mg/L～20mg/L)的塞来昔布与CDDP 1mg/L合用或塞来昔布10mg/L～20mg/L与5-Fu 0.5mg/L合用,引起拮抗作用。尼美舒利1mg/L～20mg/L与CDDP 1mg/L合用,对Caco-2细胞的抑制呈拮抗作用,而与5-Fu 1mg/L合用,对Caco-2细胞的抑制呈相加作用。结论:低浓度的塞来昔布和CDDP合用,呈相加作用,但当塞来昔布的浓度增大时,就转为拮抗。尼美舒利和CDDP合用时,无论浓度高低,都呈拮抗作用。Objective:To explore interaction of inhibiting effects of cyclooxygenase-2 (COX-2)inhibitors and anticancer drugs on human colon carcinomas cells stain,Caco-2.Methods:To observe the inhibiting action of COX-2 inhibitors and cytotoxic drugs on Caeo-2 cells by MTT assay. Results:Mter dosing 1 mg/L～10 mg/L celecoxib and CDDP (Cisplatin)1 mg/L,addition effects were observed in inhibiting action of Caco-2 strain, but antagonism was observed after dosing higher concentration of celeeoxib (15 mg/L～20 mg/L)and CCDP 1 mg/L or co-administering celecoxib 10 mg/L～20 mg/L and 5-FU 0.5 mg/L. Antagonism was observedfollowingdosingnimesulide 1 mg/L～20mg/L and CCDP to Caco-2 cell strain,whereas additivity showed after dosing nimesulide 1mg/L～20mg/L and 5-FU 1mg/L. Conchusion:Additive interactions were observed when dosing low concentration of celecoxib and CDDP to Caco-2 cell strain,and when the concentration of celecoxib was increased, the interactions turn into antagonism. Antagonism was observed when dosing nimesulide and CDDP,regardless of nimesulide concentration.

关 键 词：尼美舒利 塞来昔布 CDDP 5-Fu 相互作用 

分 类 号：R96[医药卫生—药理学]