微波技术在ELISA法检测乙肝标记物中的应用研究  

Application of the microwave technique in detecting viral B hepatitis marker by ELISA

作　　者：涂侃[1] 王启伟[2] 高玟[1] 罗庆丰[1] 

机构地区：[1]江西省肿瘤医院病理科,南昌330029 [2]江西省肿瘤医院检验科,南昌330029

出　　处：《实验与检验医学》2001年第5期262-263,300,共3页Experimental and Laboratory Medicine

摘　　要：目的将微波技术应用于ELISA法检测乙肝免疫标记物。方法对随机抽取的346例待测血清进行微波二步法标记HBsAg、HBsAb、HBeAg、HBeAb、HBcAb乙肝五项测定。同步进行ELISA常规法标记,比较结果。结果346例待测血清中微波二步法阳性率:HBsAg30.92%107/346、HBsAb24.57%85/346、HBeAg17.05%59/346、HBeAb19.36%67/346、HBcAb40.17%139/346;ELISA常规法阳性率HBsAg30.07%109/346、HBsAb25.72%89/346、HBeAg15.61%54/346、HBeAb19.65%68/346、HBcAb40.17%139/346。两组阳性结果比较无显著性差异P>0.05。结论应用微波技术进行乙肝标记物的检测具有可操作性强,重复性好,准确性高,尤其是大人缩短了实验时间,提高了工作效率,作为一种新技术,对检测乙肝免疫标记物具有一定意义。Objective To apply the microwave technique to detecting random ly the marker of viral B hepatitis by ELISA.Method Three hundred and forty six taken samples and subjected to microwave two-step labelling HBsAg HBsAb HBeAg HBeAb and HBcAb were compared with routine enzyme linked immunosorbent assay(ELISA).Result The positive rate of microwave two-step assay of346sera were:HBsAg 30.92%(107/346)、HBsAb24.57%(85/346)、HBeAg17.05%(59/346)、HBeAb19.36%(67/346)and HBcAb40.17%(139/346).The positive rate of the routine ELISA:HB-sAg30.07%(104/346)、HBsAb25.72%(89/346)、HBeAg15.61%(54/346)、HBeAb19.65%(68/346)and HBcAb40.17%(139/346).The results of the two groups were not signif-icantly different P>0.05.Conclusion The microwave technique is more operable,re-peatable and accurate,especially it can shorten the time of experiment and improve efficiency.As a new technique,it is of significanct in detecing the marker of viral B hepatitis.

关 键 词：微波 乙肝免疫标记物 抗原 抗体 

分 类 号：R446.6[医药卫生—诊断学]