聚合酶链反应-测序技术快速检测耐异烟肼结核分枝杆菌KatG基因突变的研究  

Rapid detection of the genomic mutations of INH-resistant mycobacterium tuberculosis by polymerase chain reaction-DNA sequencing

作　　者：予季[1] 熊国亮[1] 张慧慧[1] 刘珍琼[1] 

机构地区：[1]江西省胸科医院,南昌330006

出　　处：《实验与检验医学》2007年第6期549-550,568,共3页Experimental and Laboratory Medicine

基　　金：江西省卫生厅立项资助项目(20045067)

摘　　要：目的应用PCR-DNA测序技术快速检测耐异烟肼结核分枝杆菌分离株KatG基因突变,评价其在检测结核分枝杆菌异烟肼耐药性方面的应用价值。方法47株耐异烟肼结核分枝杆菌临床分离株及30株结核分枝杆菌敏感分离株用PCR-DNA测序技术检测KatG基因突变。结果47株耐异烟肼结核分枝杆菌分离株中,有31株KatG基因检出有突变,突变检出率为66.0%(31/47);30株结核分枝杆菌敏感株检出1株KatG基因突变。结论PCR-DNA测序技术方法敏感、准确、特异,可快速检测结核分枝杆菌KatG耐药基因突变,有利于耐异烟肼结核分枝杆菌耐药性的快速检测。Objective to evaluate the value of diagnosing resistance of M.tuberculosis (MTB) with KatG genes mutation analysis of INH-resistance in isolates of MTB undertaken by PCR-DNA sequencing. Methods INH resistance in 47 strains of MTB and drug susceptivity in 30 strains of MTB were detected by PCR-DNA sequencing. Results Among 47 INH-resistant strains of MTB, 31 strains had KatG gene mutations, and with 66.0%(31/47) of mutation rate. 1 of 30 drug susceptibility MTB strains had KatG gene mutation. Conclusions PCR-DNA sequencing is a seneitive, accurate and specific method for rapid detection of KatG genes mutations of MTB, and then for detecting the INH-resistant MTB.

关 键 词：分枝杆菌@结核 耐异烟肼 KATG基因 聚合酶链反应-DNA测序 

分 类 号：R446.5[医药卫生—诊断学] Q754[医药卫生—临床医学] R378.91+1[生物学—分子生物学]