ddy小鼠替代大鼠的经济简便的抗焦虑药筛选改进模型——间氯苯哌嗪诱导的小鼠明暗箱焦虑模型  

mCPP-induced Anxiety Model in Light-dark Box in ddy Mice ——A New Economic and Simple Method for Screening Anxiolytic Activity

作　　者：谭德讲[1] 武永敬明[2] 小友进 李冠民[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]日本大正制药株式会社综合研究所

出　　处：《实验动物科学》2003年第z1期65-69,共5页Laboratory Animal Science

摘　　要：目的　建立一个部分模拟临床病理的简便易得、经济有效的用于筛选和研制抗焦虑药的模型。方法　用间氯苯哌嗪 (mCPP)诱导产生焦虑 ,观察ddy小鼠和ICR小鼠在明暗箱的行为表现。结果　mCPP在SC 1- 4mg kg的剂量下即可显著降低ddy小鼠在明箱的活动次数 ,而对暗箱的活动次数影响不显著 ;1mg kg与 4mg kg组比较 ,在明箱的活动次数无显著差异 ;mCPP在SC 2 - 10mg kg的剂量下对ICR小鼠在明箱的的活动次数影响不太显著 ,只在10mg kg剂量下才显著 ;而对暗箱的活动次数却影响较大 ,在 2mg kg的剂量下即可显著降低活动次数。用安定对该模型进行验证 ,发现只用较小的样本量即可得出显著结果。结论　用ddy小鼠可以取代Wistar大鼠建立mCPP诱导焦虑的明暗箱模型 ,在样本量较小的情况下即可得到有效结果。所以是一个能够克服大鼠实验缺点的、简便易得且经济有效的用于筛选和研制抗焦虑药的良好模型。但用mCPP诱导的焦虑模型对小鼠的种属有选择性 ,ICR小鼠不适于上述模型。Objective To develop a new partially clinical mimic, economic and simple anxiety model for screening and studying anxiolytic model. Method observing the behavior activity of DDY and ICR mice in Light_Dark box after SC mCPP to induce anxiety. Result It can be significantly decrease the activity of ddy mice in light box after sc 1-4mg/kg, but the activity in dark box is not significantly changed. There is no significant difference in the light box between the 1mg/kg and the 4mg/kg group. mCPP has little effect on the activity of ICR mice in the light box, but it can significantly decrease the activity in the dark box between 2-10mg/kg. It is easy to prove the anxiolytic effect of Diazepam only in a small sample by using this model. Conclusion It is easy to get the effective result by using only small sample in the Light_Dark box after mCPP_induced anxiety in ddy mice, so we suppose it is partially clinical mimic, economic and simple anxiety model for screening and studying anxiolytic model.

关 键 词：间氯苯哌嗪[m-chlorophenylpierazine(mCPP)] 焦虑模型 ddy小鼠 ICR小鼠 

分 类 号：N[自然科学总论]