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作 者:刘云波[1] 胡树惠[2] 康爱丽[1] 王伟[1] 李惠新[1] 孙军远[3]
机构地区:[1]山东省实验动物中心 [2]济宁医学专科学校 [3]山东大学齐鲁医院,济南250002
出 处:《实验动物科学》2003年第z1期80-82,共3页Laboratory Animal Science
摘 要:能量是营养学的重要指标 ,能量摄入和消耗之间的失衡对动物的健康及保持作为实验动物的重要特性都会产生影响。对于犬的能量需要研究一般是研究基础代谢率 ,近年来研究能量需要较多 ,比如热量测定法或保持体重恒定法。保持体重恒定法是指一定时间 (5周以上 )保持体重恒定时的能量需要为主要指标 ,保持体重恒定时的进食能量即为能量需要。通过选取 1岁左右健康山东细犬 12只 ,随机分为 3组 ,每组 4只 ,各组分别喂不同能量水平的日粮 (能量水平分别为 10 2 2MJ kg、11 2 3MJ kg、11 92MJ kg) ,每天上午定时定量饲喂 ,自由饮水。每周空腹称重。饲养试验的第 15d开始收集粪便 ,连续 4d ,将 4d粪便混匀在一起 ,部分取样进行水分和含氮量测定 ,其余部分制成风干标本测定能量 ,用测热法测定能量。主要结果如下 :三组动物在不同能量水平饲喂 6周体重增加不明显(P >0 0 5 ) ;三组动物粪能和尿能无显著性差异 ,但消化能和代谢能差异显著 (P <0 0 5 )。说明对于山东细犬 ,维持体重的恒定不需要刻意控制饲料的能量水平 ,其自身可以通过不同的代谢水平调节。Energy is a very important index of the nutriology. The imbalance between the adsorption and consumption will exert influence on the animal's health and the maintenance of its characteristics as an experimental animal. The research on the energy needs of the canines is often a research of the basic metabolizing rate. Lately there are a lot of research methods, such as the heat-measuring method or the weight-maintaining method. The latter refers to a method in which we can get the energy need by using the energy need to retain the stability of the weight in a given period of time (more than 5 weeks). In this case the energy got by eating is the need of energy. By selecting 12 healthy Shandong canines of one year or so and dividing them randomly into 3 groups, with 4 in each group and then feeding each group with food of different energy level regularly at 9 in the mornings and allow them to drink as they like. Then feed them regularly in the morning with fixed amount and free drinking. Measure weighs every week in empty stomach. Collect the excrements for 4 continual days from the 15th day on, and mix them evenly, then take the samples to measure the humidity and the nitrogen contained in the samples. Make the rest into air-dry specimen to measure the energy level. The main results are summarized as follows: After 6 weeks' feeding with different energy levels, the 3 groups of animal change their weights respectively from 19 56±1 32kg to 19 28±0 89kg,20 10±0 78 kg to 20 35±1 12 kg and 19 78±1 21kg to 19 90±1 23kg,with no significant increase( P >0 05);the turd energy (1322 20±71 30, 1318 45±82 56, 1321 64±74 78 kJ/d)and urine energy(248 50±8 60, 247 68±9 10, 253 19±8 68 kJ/d)experiences no significant change, but digestion energy(3568 60±71 30, 3828 31±82 56, 4274 25±76 54 kJ/d)and metabolizing energy(3120 10±62 54, 3580 63±79 63, 4021 06±75 23 kJ/d.)witnesses great changes( P <0 05), which indicates that we need not specially control the energy level
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