微卫星DNA多态性分析在常用近交系小鼠遗传监测中的应用研究  

Microsatellite DNA Polymorphisms in Inbred Strain Mice and Selection as Genetic Monitoring Markers

作　　者：欧阳兆和[1] 陈振文[1] 李瑞生[1] 战大伟[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071

出　　处：《实验动物科学》2003年第z1期136-138,共3页Laboratory Animal Science

摘　　要：The aim of genetic monitoring is to checking the genetic contamination within inbred starains,which insures that the strains according with the require of colony . At present the methods based on allozyme biochemistry are the National Standard instructed. methods that using microsatellite DNA would be more useful for genetic monitoring than methods based on allozyme biochemistry because the genome itself is being tested rather than a protein product and a larger portion of the genome can be sampled, and easy to distinguish. methods that using microsatellite DNA had abundant microsatellite loci(over 7300,before 1999) can be identified. Applying enough microsatellite loci will present abundant straps and well polymorphism, which can reflection inherit and variation of roundly genene.In addition, this novel approach allows the rapid, sensitive,convenientand accuracy, even individual identificaton. So we should select microsatellite DNA which is polymorphisms as genetic monitoring markers to determining the strains’origin and genetic background of inbred mice. Untill now Only feasibility has been reported,and in which microsatellite DNA loci have not enough polymorphisms to distinguish genetic differences.Articles on standards and practicality have not been founded in our country. With the optimization of components of reaction buffer and amplificaton parameter,PCR for amplification microsatellite DNA was finally set up.Using the techniques microsatellite DNA can amplified efficaciously. The final concentrations of Mg 2+ was 1 .5—3.0 mmol/L,annealing temperature was 50℃—65℃.The condition for the PCR amplify were ,94℃for 3min,30cycles of 94℃ for 30s, 50℃—65℃ for 30s,72℃ for 1min,finally at 72℃ for 1min,then store at 4℃. Ten kinds of inbred strain mice including C57BL/6J,C3H/He, TA1,TA 2,615,BALB/c ,DBA/2N,129/Sv,FVB/N,AMMS/1 were investigated by PCR analysis. 14 microsatellites DNA loci on different chromosomes which we selected applied in genetic monitoring is the first time on 10 commonly uThe aim of genetic monitoring is to checking the genetic contamination within inbred starains,which insures that the strains according with the require of colony . At present the methods based on allozyme biochemistry are the National Standard instructed. methods that using microsatellite DNA would be more useful for genetic monitoring than methods based on allozyme biochemistry because the genome itself is being tested rather than a protein product and a larger portion of the genome can be sampled, and easy to distinguish. methods that using microsatellite DNA had abundant microsatellite loci(over 7300,before 1999) can be identified. Applying enough microsatellite loci will present abundant straps and well polymorphism, which can reflection inherit and variation of roundly genene.In addition, this novel approach allows the rapid, sensitive,convenientand accuracy, even individual identificaton. So we should select microsatellite DNA which is polymorphisms as genetic monitoring markers to determining the strains'origin and genetic background of inbred mice. Untill now Only feasibility has been reported,and in which microsatellite DNA loci have not enough polymorphisms to distinguish genetic differences.Articles on standards and practicality have not been founded in our country. With the optimization of components of reaction buffer and amplificaton parameter,PCR for amplification microsatellite DNA was finally set up.Using the techniques microsatellite DNA can amplified efficaciously. The final concentrations of Mg 2+ was 1 .5—3.0 mmol/L,annealing temperature was 50℃—65℃.The condition for the PCR amplify were ,94℃for 3min,30cycles of 94℃ for 30s, 50℃—65℃ for 30s,72℃ for 1min,finally at 72℃ for 1min,then store at 4℃. Ten kinds of inbred strain mice including C57BL/6J,C3H/He, TA1,TA 2,615,BALB/c ,DBA/2N,129/Sv,FVB/N,AMMS/1 were investigated by PCR analysis. 14 microsatellites DNA loci on different chromosomes which we selected applied in genetic monitoring is the first time on 10

关 键 词：微卫星DNA 近交系小鼠 遗传监测 

分 类 号：N[自然科学总论]