近交系大鼠DNA指纹图和微卫星DNA多态性的分析  

Analysing Polymorphism of DNA Fingerprints and Microsatellite DNA in Inbred Rats

作　　者：李瑞生[1] 陈振文[1] 欧阳兆和[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071

出　　处：《实验动物科学》2003年第z1期139-141,共3页Laboratory Animal Science

摘　　要：Monitoring method of biochemical markers is inferred corresponding gene changed according to protein changed in laboratory animals, and have definite limitation in some extent. But DNA fingerprints and microsatellite DNA directly monitored DNA of genetic materials, more accurate、more reliable and direct than biochemical markers. In the experiments six domestic strains(SHR、SHR/sp、WKY、LEW、RCS、F344) of inbred rat were analyzed DNA polymorphism using DNA fingerprints and microsatellite DNA, and set up the exact、reliable、speedy、convenient method of in the heredity monitoring of inbred rats. The result indicated:(1)DNA fingerprints: ①There were 14-19 distinguishable bands on 4.0kb-23.1kb every DNA fingerprinting. The similarity coefficient(F) and the probability of band sharing(X) were more than 0.9 in different individual of the identical inbred rat strain. and indicated more high similarity in genetic materials of the various individual in same group rats. ②The similarity coefficient and the probability of band sharing of DNA fingerprinting were less than 0.07, probability of the identical DNA fingerprinting was less than 10 -22 among the different individuals of different inbred strain. and indicated that there was more different in genetic materials of various individual in different inbred rat groups. ③)DNA fingerprinting obtained by four times experiment from same individual genome are almost coincident DNA fingerprinting from various tissues are also coincident in the same individual of 6 inbred rat strains. ④DNA fingerprinting of filial generation which RCS and LEW hybridized is analyzed, whose bands are found in their parents bands and complied with Mendel heredity law. (2) Microsatellite DNA:①DNA polymorphism was studied using microsatellite DNA method in 6 inbred rat strains. There is much polymorphism among the various rat strains;It was coincidence among the same inbred strains rat groups. ②10 microsatellite loci are analyzed in 6 inbred rat strains group. and the similarity cMonitoring method of biochemical markers is inferred corresponding gene changed according to protein changed in laboratory animals, and have definite limitation in some extent. But DNA fingerprints and microsatellite DNA directly monitored DNA of genetic materials, more accurate、more reliable and direct than biochemical markers. In the experiments six domestic strains(SHR、SHR/sp、WKY、LEW、RCS、F344) of inbred rat were analyzed DNA polymorphism using DNA fingerprints and microsatellite DNA, and set up the exact、reliable、speedy、convenient method of in the heredity monitoring of inbred rats. The result indicated:(1)DNA fingerprints: ①There were 14-19 distinguishable bands on 4.0kb-23.1kb every DNA fingerprinting. The similarity coefficient(F) and the probability of band sharing(X) were more than 0.9 in different individual of the identical inbred rat strain. and indicated more high similarity in genetic materials of the various individual in same group rats. ②The similarity coefficient and the probability of band sharing of DNA fingerprinting were less than 0.07, probability of the identical DNA fingerprinting was less than 10 -22 among the different individuals of different inbred strain. and indicated that there was more different in genetic materials of various individual in different inbred rat groups. ③)DNA fingerprinting obtained by four times experiment from same individual genome are almost coincident DNA fingerprinting from various tissues are also coincident in the same individual of 6 inbred rat strains. ④DNA fingerprinting of filial generation which RCS and LEW hybridized is analyzed, whose bands are found in their parents bands and complied with Mendel heredity law. (2) Microsatellite DNA:①DNA polymorphism was studied using microsatellite DNA method in 6 inbred rat strains. There is much polymorphism among the various rat strains;It was coincidence among the same inbred strains rat groups. ②10 microsatellite loci are analyzed in 6 inbred rat strains group. and the

关 键 词：近交系大鼠 DNA指纹图 微卫星DNA 

分 类 号：N[自然科学总论]