家蚕生物反应器与家蚕实验动物化饲育技术  

The Domestic Silkworm Bioreactor and Silkworm Experiment Animalization Breeding Techniques

作　　者：刘金明[1] 傅志强[1] 林矫矫[1] 田锷[1] 蔡幼民[1] 桂仲争[2] 庄大桓[2] 吴祥甫[3] 刘瑞三[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室,上海200232 [2]中国农业科学院蚕业研究所,镇江212018 [3]中国科学院上海生物化学研究所,上海200031

出　　处：《实验动物科学》2003年第z1期149-150,共2页Laboratory Animal Science

摘　　要：By using the domestic silkworm as the bioreactor (reorganizing the nucleus polygon virus expression system) to produce genetic products, we can attain our goal with less cost, higher productivity and activity in a safe manner. With the swelling development of biosciences and biotechnologies in recent years, we should develop new experimental species, especially the lower species, to replace the mammals and explore the animals’ potential as a resource to benefit the human beings. We will make a detailed account of what we have achieved by utilizing the domestic silkworm exotic genes and the progress we have made in its animalization breeding technologies. Our domestic silkworm expression system is composed of its cells, transferring carrier, modified BmNPV, its chrysalis. We have obtained the rsj14NPV, which contains Japanese schistosome fatty acid albumen genes, and rsj28NPV, which contains Japanese schistosome 8KDGST genes. After being infected by the rsj14NPV, every silkworm (871×872) can produce 3.5 mgs’ purified albumen. After the silkworm 57A.57B×24.26 is infected with Rsj28NPV, every silkworm will turn out 5mgs’ purified albumen. By employing rSj14NPV(871×872),we observe the relations between the volume of contracted virus, the timing of the contraction, the duration of the virus and the amount of the produced albumen. The results are as follows: the silkworm that is inoculated with 4μL and 6μL produces 4-5mgs/silkworm, which is the highest, while the silkworm that is inoculated with 1μL group and 2μL group turns out only 1 mg, which is the lowest; the timing of the inoculation has no significant influence on the productivity; the reorganized virus that is preserved for a long period of time(9 months)is not very productive after being inoculated, which shows that we should use the required resurrected reorganized virus to achieve high productivity; by inoculating 55μLs’ resurrected reorganized virus(rsj14NPV) into the silkworm 871×872 will produce 4-5 mgs’ albumen, which is similar to thaBy using the domestic silkworm as the bioreactor (reorganizing the nucleus polygon virus expression system) to produce genetic products, we can attain our goal with less cost, higher productivity and activity in a safe manner. With the swelling development of biosciences and biotechnologies in recent years, we should develop new experimental species, especially the lower species, to replace the mammals and explore the animals' potential as a resource to benefit the human beings. We will make a detailed account of what we have achieved by utilizing the domestic silkworm exotic genes and the progress we have made in its animalization breeding technologies. Our domestic silkworm expression system is composed of its cells, transferring carrier, modified BmNPV, its chrysalis. We have obtained the rsj14NPV, which contains Japanese schistosome fatty acid albumen genes, and rsj28NPV, which contains Japanese schistosome 8KDGST genes. After being infected by the rsj14NPV, every silkworm (871×872) can produce 3.5 mgs' purified albumen. After the silkworm 57A.57B×24.26 is infected with Rsj28NPV, every silkworm will turn out 5mgs' purified albumen. By employing rSj14NPV(871×872),we observe the relations between the volume of contracted virus, the timing of the contraction, the duration of the virus and the amount of the produced albumen. The results are as follows: the silkworm that is inoculated with 4μL and 6μL produces 4-5mgs/silkworm, which is the highest, while the silkworm that is inoculated with 1μL group and 2μL group turns out only 1 mg, which is the lowest; the timing of the inoculation has no significant influence on the productivity; the reorganized virus that is preserved for a long period of time(9 months)is not very productive after being inoculated, which shows that we should use the required resurrected reorganized virus to achieve high productivity; by inoculating 55μLs’ resurrected reorganized virus(rsj14NPV) into the silkworm 871×872 will produce 4-5 mgs' albumen, which is similar to that of a

关 键 词：家蚕 生物反应器 实验动物化 

分 类 号：N[自然科学总论]