Mechanism of the Effect of Guzhen Recipe (固真方) on Regulating Immune Function in Senile Rats  

作　　者：刘 颖 赵伟康 国琴 凌昌全 

出　　处：《Chinese Journal of Integrative Medicine》2000年第3期204-207,共4页中国结合医学杂志（英文版）

摘　　要：Objective: To explore the effect of Guzhen recipe (固真方, GZR) on mRNA expression of interleukin-2 (IL-2) and interleukin-6 (IL-6) in senile rats as well as the relationship between dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) and the immune function regulation of GZR. Methods: Northern Blot and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the mRNA expression of IL-2 and IL-6, meanwhile coating-antibody-counting method was used to detect DHEA(S) level in serum. Results: Compared with the young group, the splenocyte mRNA expression of IL-2 in senile rats was lowered and that of IL-6 was increased along with the decrease of serum DHEA(S) level. GZR, however, could improve the above indices in the senile rats. Conclusion: GZR might regulate the immune function of aged rats through increasing the serum level of DHEA(S).Objective: To explore the effect of Guzhen recipe (固真方, GZR) on mRNA expression of interleukin-2 (IL-2) and interleukin-6 (IL-6) in senile rats as well as the relationship between dehydroepiandrosterone (DHEA) and its sulfate (DHEAS) and the immune function regulation of GZR. Methods: Northern Blot and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the mRNA expression of IL-2 and IL-6, meanwhile coating-antibody-counting method was used to detect DHEA(S) level in serum. Results: Compared with the young group, the splenocyte mRNA expression of IL-2 in senile rats was lowered and that of IL-6 was increased along with the decrease of serum DHEA(S) level. GZR, however, could improve the above indices in the senile rats. Conclusion: GZR might regulate the immune function of aged rats through increasing the serum level of DHEA(S).

关 键 词：Guzhen recipe interleukin-2 mRNA interleukin-6 mRNA dehydroepiandrosterone (sulfate) 

分 类 号：R285[医药卫生—中药学]