HBV免疫标志物及DNA检测结果分析  

The Result Analysis Between HBV Immune Marks and HBV DNA

作　　者：欧阳淑英 何准发[2] 

机构地区：[1]永兴县第二人民医院检验科,湖南永兴423301 [2]郴州市第一人民医院中心医院检验科,湖南郴州423000

出　　处：《医学检验与临床》2008年第3期45-46,共2页Medical Laboratory Science and Clinics

摘　　要：目的通过了解HBV免疫标志物(HBVM)与HBV DNA的相互关系,为临床诊断和治疗乙肝提供可靠的依据。方法收集1156例受检者临床血清标本,同时采用酶联免疫吸附试验检测HBVM和荧光定量PCR检测HBV DNA,比较HBVM不同血清模式HBV DNA检出率及其含量,分析HBVM与HBV DNA之间的关系。结果血清HBV DNA阳性检出率及其量与HBVM有关,HBsAg、HBeAg抗HBc阳性血清模式组与其它各组比较,差异有显著性(P<0.01)。结论HBeAg与HBV DNA含量明显相关;HBeAg阴性不能代表病毒复制停止。Objective By observing the relation between HBV immune marks and HBV DNA,so we can provide reliable evidence for clinical diagnosing and curing HBV.Methed 1156 blood serum samples were collected from the clinic.HBV immune marks were tested by ELEISA and HBV DNA was tested by FQ-PCR.Then we analysed the relation between different HBV immune mark patterns and HBV DNA concentration.Results the positive rate of HBV DNA had some relation with HBV immune mark.There was evident difference compared HBsAg positive group、HBeAg and HBC antibody positive group with other groups.Conclusion HBeAg had evident relation with HBV DNA concentration;the negative of HBeAg was not a symbol of HBV stopping duplication.

关 键 词：乙型肝炎病毒 免疫标志物 脱氧核糖核酸 酶联免疫吸附试验 荧光定量聚合酶链反应技术 

分 类 号：R446.6[医药卫生—诊断学]