系统性红斑狼疮患者th1/th2失衡的研究  

Imbalance of Th1/Th2 in recent-onset systemic lupus erythematosus patients

作　　者：贺仆[1] 孙涛[1] 

机构地区：[1]山东省千佛山医院,济南250014

出　　处：《医学检验与临床》2008年第3期8-11,17,共4页Medical Laboratory Science and Clinics

摘　　要：目的探讨系统性红斑狼疮(SLE)病人th1/th2的失衡状况。方法利用三荧光标记法流式细胞术检测抗细胞表面抗原,抗细胞因子。检测出CD4+干扰素(IFN)-γ+白细胞介素(IL)4-及CD4+IFN-γ-IL-4+细胞作为Thl及Th2细胞,从单细胞水平研究初发SLE患者Thl/Th2平衡。结果未用药治疗的SLE病人其Thl细胞明显低于正常对照组(P=0.02)。31例SLE患者按Thl/Th2可分成两组,Thl/Th2降低组与Thl/Th2升高组。Thl/Th2降低组其Thl细胞的降低十分明显。Thl/Th2升高组中Thl细胞仅轻度下降。结论Thl型反应与Th2型反应均参与了SLE的发病。不同患者可能免疫系统的异常并不同。Objective To detect intracellular cytokines of systemic lupus erythematosus(SLE) patients untreated with glucocorticoids and immunosuppressive agents using three-color flow cytometry.Methods Whole blood were cultured and stained with.Cy-conjugated anti-surface antigen monoclonal antibody(mAb).Then fixation and permeabilization method were used to perform intracellular cytokin staining with FITC-conjugated IFN-γ specific mAb and PE-conjugated IL-4 specific mAb.The percentage of IFN-γ and IL-4 positive cells was counted by FACS,and the Thl/Th2 balance was evaluated by the ratio of IFN-γ to IL-4 Results The percentage of Thl-type cells in untreated SLE patients was significantly lower than that in healthy controls(P=0.02).Patients were divided into two groups:one with high Thl/Th2 ratio and another with low Thl/Th2 ratio.Patients with high Thl/Th2 ratio had more frequent malar rash(P=0.036).Conclusion The results indicate that both Thl and Th2 responses play prominent roles in the pathogenesis of lupus.The decrease of Thl cells may relate to malar rash.

关 键 词：红斑狼疮 系统性 THL细胞 TH2细胞 细胞因子 干扰素Ⅱ型 白细胞介素4 

分 类 号：R593.241[医药卫生—内科学]