药用百合多糖提取纯化工艺的研究  

Study on the Extraction and Purification of Lily Polysaccharide

作　　者：何纯莲[1] 张小艳[1] 杨球桢[1] 杨小红[1] 

机构地区：[1]湖南师范大学医学院,湖南长沙410006

出　　处：《湖南师范大学学报（医学版）》2008年第3期7-9,12,共4页Journal of Hunan Normal University(Medical Sciences)

基　　金：湖南省科技厅资助科研项目(06FJ3182);湖南省教育厅资助科研项目(06C497)

摘　　要：目的:优化百合多糖提取、纯化工艺。方法:以水提醇沉法提取百合多糖,savage法去蛋白纯化。在单因素实验的基础上,采用正交试验的方法对影响百合多糖提取、纯化的因素进行优化。结果:百合多糖的最佳提取工艺为:在65℃下,用15倍药材量的蒸馏水提取3次,每次4h。最佳纯化工艺为:样品:氯仿(v∶v)为3∶2,氯仿:正丁醇为2∶1,振摇时间为15min。结论:优化的百合多糖提取纯化工艺简单、稳定,易于产业化规模生产。Objective The extraction and purification technological condition of lily polysaccharides were optimized.Methods Using the water extraction ethanol precipitation method extract lily polysaccharides,savage method removing proteins.On the basis of single-factor experiments,the extraction and purification teacnological conditions of polysaccharides from lily were optimized by vertical-method.Results The extraction technical of lily polysaccharides is selected as following :15 times of distilled water as the so...

关 键 词：百合多糖 提取 纯化 正交实验 

分 类 号：R284.2[医药卫生—中药学]