Studies of poly-L-lysine-starch nanoparticle preparation and its application as gene carrier  

作　　者：XIAO Suyao LIU Xuanming TONG Chunyi LIU Jun TANG Dongying ZHAO Lijian 

机构地区：[1]Institute of Life Science and Biotechnology, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China Institute of Life Science and Biotechnology, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China Institute of Life Science and Biotechnology, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China Institute of Life Science and Biotechnology, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China Institute of Life Science and Biotechnology, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China Institute of Life Science and Biotechnology, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China

出　　处：《Science China Chemistry》2005年第2期162-166,共5页中国科学（化学英文版）

摘　　要：Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in wa- ter-in-oil microemulsion, with soluble starch as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we devel- oped the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection effi- ciency, low cell toxicity and biodegradability.Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in wa- ter-in-oil microemulsion, with soluble starch as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we devel- oped the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection effi- ciency, low cell toxicity and biodegradability.

关 键 词：ANION STARCH nanoparticle  poly-L-lysine  non-virus gene carrier  cell transfection. 

分 类 号：N[自然科学总论]