Distinguishing malignant from normal stomach tissues and its in vivo, in situ measurement in operating process using FTIR Fiber-Optic techniques  

作　　者：XU Yizhuang1, YANG Limin1, XU Zhi3, ZHAO Ying2, LING Xiaofeng3, LI Qingbo1, WANG Jiansheng4, ZHANG Nengwei3, ZHANG Yuanfu1 & WU Jinguang1 1. The State Key Laboratory of Rare Earth Materials and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China 3. Department of Surgery, Third Hospital, Peking University, Beijing 100083, China 4. Department of Oncology Surgery, the First Hospital of Xi’an Jiaotong University, Xi’an 710061, China Correspondence should be addressed to Wu Jinguang (email: [e-mail]) 

出　　处：《Science China Chemistry》2005年第2期167-175,共9页中国科学（化学英文版）

基　　金：the Nat ional Natural Science Foundation of China(Grant Nos.30371604,39730160 , 50203001);the State Key Project for Fundamental Research of MOST(2002CCA01900); Project of Education Ministry Ph.D Candidate Program.

摘　　要：Gastric tissue samples were studied using mid-IR fiber-optic attenuated total reflectance (ATR) spectroscopy. FTIR spectra of 90 tissue samples from 48 patients, including 32 normal and 58 malignant tissue samples, were chosen as examples. Malignancy was usually characterized by the absence of CH and C=O bands, a weak amide II band near 1545 cm-1, a shift of the amide I band to lower wave number, a decrease in the ～1450 cm-1 peak to less than the～1400 cm-1 peak. Subtraction spectra indicate that the amide I and amide II bands of normal and malignant tissues have larger differences in peak positions and relative intensities. The statistical analysis results confirm this conclusion. The results indicate that FTIR fiber optic techniques provide important information about cancerous tissue of the stomach, which can be used to differentiate the malignant tissue from the normal tissue. Based on the above results we successfully realize the detection of the tumor tissues of digestive tract in vivo and in situ. And the results of detection cancer near operating room and in vivo and in situ in the operating room are consistent with the conclusions for the samples stored in liquid N2, which is the basis for the clinical application.Gastric tissue samples were studied using mid-IR fiber-optic attenuated total re- flectance (ATR) spectroscopy. FTIR spectra of 90 tissue samples from 48 patients, including 32 normal and 58 malignant tissue samples, were chosen as examples. Malignancy was usually characterized by the absence of CH and C=O bands, a weak amide II band near 1545 cm?1, a shift of the amide I band to lower wave number, a decrease in the ~1450 cm?1 peak to less than the ~1400 cm?1 peak. Subtraction spectra indicate that the amide I and amide II bands of normal and malignant tissues have larger differences in peak positions and relative intensities. The sta- tistical analysis results confirm this conclusion. The results indicate that FTIR fiber optic tech- niques provide important information about cancerous tissue of the stomach, which can be used to differentiate the malignant tissue from the normal tissue. Based on the above results we suc- cessfully realize the detection of the tumor tissues of digestive tract in vivo and in situ. And the results of detection cancer near operating room and in vivo and in situ in the operating room are consistent with the conclusions for the samples stored in liquid N2, which is the basis for the clinical application.

关 键 词：FTIR  Fiber-Optics  STOMACH tissues  cancer  diagnosis. 

分 类 号：R656.6[医药卫生—外科学]