复方中药卫康胶囊抗氧化作用的实验研究  

Anti-oxidant effect of Vigconic Ⅵ-28 capsule, a formulated traditional Chinese medicine, in BALB/c mice

作　　者：胡刚[1] 张岩[2] 徐晓军[2] 

机构地区：[1]卫生部北京老年病研究所,100730 [2]北京大学医学部免疫学系,100083

出　　处：《中国老年保健医学》2005年第2期42-44,46,共4页Chinese Journal of Geriatric Care

摘　　要：背景卫康星牌卫康胶囊是以人参、鹿茸、菟丝子等名贵中药为主要成分提取的现代复方中药,具有增强免疫力的功能。以往的人体试验研究发现此产品的栓剂型具有明显的抗氧化作用。目的研究卫康星牌卫康胶囊在动物实验中的抗氧化作用及其机制。方法纯系BALB／c小鼠喂食混有卫康胶囊药粉或者维生素E的饲料。至28天时取小鼠血液和脑、肝、心肌和骨骼肌等组织, 测定锰过氧化物歧化酶活性和还原型谷胱甘肽水平分析其线粒体抗氧化状态,通过测定ATP产生能力确定其线粒体功能。结果喂食含有卫康胶囊药粉或者维生素E饲料的BALB／c小鼠血液以及脑、肝、肌肉中线粒体抗氧化状态明显提升,线粒体功能得到加强。雄性与雌性动物之间未见明显区别。有些情况下卫康胶囊的作用强于维生素E。结论卫康星牌卫康胶囊可以提高小鼠不同组织中线粒体抗氧化状态,促进线粒体功能,具有明显的抗氧化功效。Background Previous studies have shown that treatment with Vigconic Ⅵ - 28 capsule (Ⅵ-28), a formulated traditional Chinese medicine containing radix Ginseng, cornu cervi Pantotrichum and semen Cuscutae, could enhance red cell antioxidant capacity in male human subjects. Aims To determine whether treatment with Ⅵ-28 could enhance mitochondrial antioxidant and functional status in various tissues of mice. Methods Groups of BALB/c mice were fed on normal mouse food, or food containing 0.5% or 2% Ⅵ-28, or vitamin E. By day 28, the animals were sacrificed for blood samples and tissues of the brain, heart, liver, and skeletal muscle. Mitochondrial antioxidant and functional status were assessed. Results Ⅵ-28 and Vitamin E treatment increased red cell CuZn - SOD activity. Ⅵ-28 treatment could enhance the mitochondrial antioxidant status in various tissues to a larger extent than those of vitamin E treatment, as evidenced by the increase of Mn - SOD activity, ot - TOC concentration and GSH. The enhancement of mitochondrial antixoidant status afforded by Ⅵ-28 treatment was associated with the stimulation in mitochondrial ATP - GC. Conclusion Treatment with Ⅵ-28 could enhance mitochondrial antioxidant and functional status in vivo.

关 键 词：卫康胶囊 抗氧化 线粒体 

分 类 号：R285[医药卫生—中药学]