噬菌体抗体库技术及其在水产养殖的应用前景  

Progress of phage antibody library technique and its application prospect in aquaculture

作　　者：章晋勇[1] 吴英松[1] 汪建国[1] 

机构地区：[1]中国科学院水生生物研究所,湖北武汉430072

出　　处：《水产学报》2004年第3期329-333,共5页Journal of Fisheries of China

基　　金：国家自然科学基金资助项目(30271028);中国科学院知识创新工程课题(KSCX2-1-04)

摘　　要：Phage display antibody library has been proven to be a powerful technique used in development of antibodies. Since it was established in 1990, the technology has made enormous improvement and played more and more important role in basic research of biology, immunology, oncology, protein engineering, ligand-receptor studies and proteomics among others in last two decades while there is no report about the application of it in aquaculture so far. It is a success implication of phage display technique in antibody engineering in which antibodies or antibody fragments are displayed on the surface of filamentous bacteriophage by genetic fusion to a coat protein of phage. Cooperating with the effective screening technique, affinity panning, these form the principle of phage display antibody library. The most characteristic of it is a direct physical link between phenotype and genotype. So, the technology makes it practicable to improve characteristic of selected antibodies by genetic manipulation. In the present work, the background, principle and advantages of the powerful tool over traditional hybridroma technolgy are summarized. In addition, several key problems possible to face in the course of application of the technology, including improving the diversity of library, augmentation of library size, generation of high affinity antibody and effective screening of specific antibody were dissertated. At last the possible implication prospect of phage display antibody technique in aquaculture was discussed, especially in elucidating the immune system of fish and producing large amount antibodies with important diagnostic and therapeutic value in fish diseases.Phage display antibody library has been proven to be a powerful technique used in development of antibodies. Since it was established in 1990, the technology has made enormous improvement and played more and more important role in basic research of biology, immunology, oncology, protein engineering, ligand-receptor studies and proteomics among others in last two decades while there is no report about the application of it in aquaculture so far. It is a success implication of phage display technique in antibody engineering in which antibodies or antibody fragments are displayed on the surface of filamentous bacteriophage by genetic fusion to a coat protein of phage. Cooperating with the effective screening technique, affinity panning, these form the principle of phage display antibody library. The most characteristic of it is a direct physical link between phenotype and genotype. So, the technology makes it practicable to improve characteristic of selected antibodies by genetic manipulation. In the present work, the background, principle and advantages of the powerful tool over traditional hybridroma technolgy are summarized. In addition, several key problems possible to face in the course of application of the technology, including improving the diversity of library, augmentation of library size, generation of high affinity antibody and effective screening of specific antibody were dissertated. At last the possible implication prospect of phage display antibody technique in aquaculture was discussed, especially in elucidating the immune system of fish and producing large amount antibodies with important diagnostic and therapeutic value in fish diseases.

关 键 词：噬菌体抗体库 应用 前景 水产养殖 

分 类 号：Q782[生物学—分子生物学] S917[农业科学—水产科学]