45株MRSA消毒剂的最小杀菌浓度和耐消毒剂基因的检测  

DETECTION OF THE MBC TO DISINFECTANTS AND DISINFECTANT-RESISTANT GENE IN FORTY-FIVE STRAINS OF MRSA

作　　者：孙静娜[1] 张征[1] 武艳[1] 张卫国[2] 高志成[3] 代丽丽[1] 

机构地区：[1]河北医科大学第一医院检验科,河北石家庄050031 [2]河北医科大学期刊社,河北石家庄050017 [3]河北省保定市第二医院药剂科,河北保定071051

出　　处：《河北医科大学学报》2009年第8期804-806,共3页Journal of Hebei Medical University

基　　金：河北省科学技术研究与发展指导计划(052761136);河北省医学科学研究重点课题计划(06197)

摘　　要：目的了解耐甲氧西林金黄色葡萄球菌(methicillin-resistant staphylococcus aureus,MRSA)对洗必泰、新洁尔灭消毒剂的耐药性和消毒剂耐药基因的检出情况。方法收集2007年1月—2008年6月分离自河北医科大学第一医院临床患者的MRSA 45株,采用稀释中和法测定细菌对洗必泰、新洁尔灭、碘伏的最小杀菌浓度;采用聚合酶链式反应对45株MRSA的消毒剂耐药基因qacA进行检测。结果 MRSA对洗必泰、新洁尔灭消毒剂产生了抗药性,消毒剂耐药基因qacA的检出率为40%。结论 MRSA对洗必泰、新洁尔灭等临床常用消毒剂多重耐药,消毒剂耐药基因qacA是MRSA对消毒剂耐药的重要机制。Objective To investigate the disinfectants-resistant genes and resistance in methicillin-resistant staphylococcus aureus (MRSA) to chlorhexidine and benzalkonium bromide disinfectants.Methods To collect 45 strains of MRSA isolated from the first hospital of Hebei medical university between Jan 2007 and the next June.Bacterial minimum bactericidal concentration(MBC) of chlorhexidine and benzalkonium bromide were performed by average; the disinfectants-resistant qacA gene's of 45 strains of MRSA were detected by polymerase chain reaction(PCR).Results Resistance was produced to chlorhexidine and benzalkonium bromide disinfectants by MRSA,detectable rate of disinfectants-resistant qacA genes was 40%.Conclusion MRSA is multiresistant to chlorhexidine and benzalkonium bromide disinfectants,disinfectants-resistant qacA genes are important for the resistance of MRSA to disinfectants.

关 键 词：葡萄球菌 金黄色 抗药性 聚合酶链反应 

分 类 号：R378.1[医药卫生—病原生物学]