光度法测定青稞秸秆中蛋白质的含量  

The Photometric Method for the Determination of Protein Content in Barley Straw

作　　者：于军[1] 朵兴红[1] 宋萍[1] 

机构地区：[1]青海民族大学化学系,青海西宁810007

出　　处：《青海师范大学学报（自然科学版）》2009年第3期61-64,共4页Journal of Qinghai Normal University(Natural Science Edition)

基　　金：国家教育部"春晖计划"科研合作项目(编号Z2005-2-81002)部分内容

摘　　要：本文对凯氏定氮法进行了改进,减少了蒸馏氨气的步骤,避免了氨气的损失和滴定的误差,采用分光光度法测定青稞秸秆消煮液与奈氏试剂进行显色反应后的供试液,测出氮含量,将结果乘上换算系数6.25,计算出粗蛋白的含量.测量条件:采用双波长分光光度法,在波长349nm和339nm进行测量,回归方程:△A=-0.0152C+0.0566,r=0.9998(NH3-N的线性范围:0.1μg/mL-20μg/mL).we improved method of Kjeldahl nitrogen determination,simplified the step of distill ammonia,avoided loss and reduced titration error of ammonia,through photometric method for determination of the test fluid of coloured complex for barley straw.Determination of the nitrogen content,multiplied by 6.25,counted the protein content in barley straw.survey conditions: use double wavelength of the photometric method,determination at 349nm and 339nm,recurrence equation: △A=-0.0152C + 0.0566,r=0.9998(linear scope of determination NH3-N:0.1μg/mL^20μg/mL).

关 键 词：光度法 奈氏试剂 青稞秸秆 蛋白质 含量 

分 类 号：O657.32[理学—分析化学] S546[理学—化学]