丹参合参麦注射液对肺纤维化大鼠的干预作用及对TGF-β_1表达的影响  

INTERVENE FUNCTION OF DANSHEN ANE SHENMAI INJECTION ON RATS'PULMONARY FIBROSIS AND EFFECTS ON TGF-β_1 EXPRESSION

作　　者：王新华[1] 朱渊红[1] 王真[1] 许励[2] 

机构地区：[1]浙江中医药大学附属第一医院,杭州310006 [2]浙江省人民医院,杭州310000

出　　处：《中国中医药科技》2009年第6期441-442,424,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省中医管理局资助项目No.2002C017

摘　　要：目的:观察丹参合参麦注射液对肺间质纤维化的治疗作用并探讨其作用机理。方法:采用BLMA5致大鼠肺间质纤维化的造模方法。随机将72只大鼠分为:空白组、模型组、丹参组、丹参合参麦组4组,并分别给药,各组分别于第7、14、28天各处死6只大鼠,依次收集支气管肺泡灌洗液,取肺病理标本作HE和TGF-β1免疫组化染色。通过计算机图像分析系统定量分析第28天的病理改变,免疫组化图像分析测量第7、14、28天的TGF-β1吸光度,以及ELISA酶法测定支气管肺泡灌洗液中第7、14、28天的TGF-β1含量。结果:经丹参和丹参合参麦注射液干预后,大鼠的纤维化和肺泡炎均较模型组轻,丹参合参麦组比丹参组更轻(P<0.05)。丹参组和丹参合参麦组的肺组织TGF-β1平均吸光度、肺泡灌洗液的TGF-β1的含量均较模型组低(P<0.01),其中第14天丹参合参麦组较丹参组更低(P<0.05)。结论:以丹参合参麦注射液为代表的益气养阴、活血化瘀法对肺纤维化有明显改善作用,其作用机理与其降低TGF-β1的表达有关。Objective:To observe the effects of danshen and shenmai injection on pulmonary fibrosis rats and explore the mechanism.Methods:The models of pulmonary fibrosis were made by injecting bleomycin to trachea. 72 rats were randomly divided into 4 groups,normal,model,danshen,and danshen with shenmai groups,each group was given corresponding medicine,6 rats of each group were killed on the 7th,14th and 28th day,the BALF was collected and the pulmonary tissue was incised to make pathological sections which were sta...

关 键 词：肺纤维化/中医药疗法 丹参注射液/治疗应用 参脉注射液/治疗应用 大鼠 

分 类 号：R285.5[医药卫生—中药学]