肠炎清通过抑制NF-κB活性对大鼠肠黏膜起抗炎作用  

Changyanqing decoction produces anti-inflammatory effect by inhibiting the activation of nuclear factor-κB

作　　者：宋卫兵[1] 吕永慧[2] 李亚南[1] 肖丽萍[1] 余新沛[1] 刘岗[1] 王媛媛[3] 张小兰[3] 李鹰飞[3] 

机构地区：[1]广州军区广州总医院干部二科,广东广州510010 [2]广州市市中医医院,广东广州510000 [3]南方医科大学南方医院消化病研究所,广东广州510005

出　　处：《南方医科大学学报》2009年第7期1431-1434,共4页Journal of Southern Medical University

摘　　要：目的检测大鼠小肠炎模型肠黏膜通透性的改变,探讨肠炎清对大鼠小肠炎模型肠黏膜通透性的作用的机制。方法应用氨甲碟呤制备大鼠小肠炎模型,实验设正常对照组、模型对照组、N-乙酰半胱氨酸(NAC,100mg/kg)组及肠炎清组(CYQ,100mg/kg),每天灌胃给药1次,共6d。每天观察大鼠疾病活动指数(DAI)和肠黏膜损伤指数(CMDI),光镜下观察组织学改变并评分(HS)。ELISA测定大鼠IL-10,RT-PCR方法检测大鼠肠黏膜内TNF-α、IL-1β的表达,应用Western blotting测定各处理组细胞内磷酸化胞质IκB蛋白表达水平。结果与正常组相比,小肠炎模型组DAI、CMDI、HS评分明显升高(P<0.01)。肠炎清组DAI,CMDI,HS评分较模型组有明显下降(P<0.01)。MTX组TNF-α、IL-1βmRNA达水平均较正常对照组明显增高,肠炎清组与NAC治疗组TNF-α、IL-1β表达水平低于MTX组,但IL-10的表达高于MTX组。肠炎清能抑制IκB的降解。结论姜黄素对肠黏膜起保护作用的机制可能是抑制NF-κB活性,进而减少致炎细胞因子TNF-α、IL-1βmRNA表达。Objective To detect the changes in intestinal mucosal permeation in rats with methotrexate-induced small intestinal damage and investigate the protective effects of Changyanqing decoction.Methods Rat enteritis model was established by methotrexate(MTX) and sodium chloride.The rats were randomly divided into normal control group,model group,N-acetylcysteine(NAC) group and Changyanqing decoction group,and Changyanqing decoction(100 mg/kg) or saline was administered daily in the corresponding groups by gastric...

关 键 词：肠炎清 小肠炎 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]