甘草提取液对肠粘膜P-糖蛋白的作用  

Effect of liquorice decoction on rat intestinal P-glycoprotein

作　　者：姚红卫[1] 傅晓幼[1] 解庆东[1] 黄蓓蓓[1] 孙亚彬[1] 李国锋[1] 

机构地区：[1]南方医科大学南方医院,广东广州510515

出　　处：《南方医科大学学报》2009年第8期1571-1573,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(C03050205)

摘　　要：目的研究甘草提取液对肠粘膜P-糖蛋白(P-gp)的调控作用。方法通过体外Ussing chamber实验,研究甘草提取液对P-gp底物罗丹明123(R123)转运的直接影响,以及甘草灌胃后对R123和荧光素钠(CF)经空肠粘膜透过性的影响。结果甘草提取液直接作用于肠粘膜时对R123吸收方向和分泌方向的影响无显著差异;灌胃相当原生药10g·Kg-1的甘草提取液每天2次,1周后,可显著提高R123吸收方向的转运,R123分泌方向有增加趋势,而对CF经空肠粘膜吸收和分泌方向的透过性均无显著影响。结论甘草可能是P-gp的轻度抑制剂,通过抑制存在于肠粘膜的P-gp活性而发挥其增加底物药物R123吸收的能力。Objective To investigate the effect of liquorice in functional modulation of intestinal P-glycoprotein (P-gp) in rats. Methods An in vitro diffusion chamber system (Ussing chamber) was used to examine the direct effect of liquorice decoction on rhodamine 123 (a subtrate of P-gp) transport and evaluate the permeability of rhodamine 123 or fluorescein sodium through rat jejunum membranes after oral administration of liquorice decoction. Results Direct application of liquorice decoction did not obviously affec...

关 键 词：甘草提取液 P-糖蛋白 罗丹明123 Ussing CHAMBER 透过性 

分 类 号：R285.5[医药卫生—中药学]