抗小鼠TLR2胞外段单表位抗体TSP-2对酵母多糖诱发小鼠腹膜炎的影响  

Effect of TSP-2 antibody against a single epitope of mouse Toll-like receptor 2 extracellular domain on zymosan A-induced peritonitis in mice

作　　者：杨翠兰[1] 赵文忠[2] 黄恩平[1] 罗深秋[1] 

机构地区：[1]南方医科大学基础医学院细胞生物学教研室,广东广州510515 [2]广东省计划生育科学技术研究所,广东广州510600

出　　处：《南方医科大学学报》2009年第8期1521-1524,共4页Journal of Southern Medical University

基　　金：国家青年自然科学基金(30801032)

摘　　要：目的观察抗小鼠TLR2胞外段(mTLR2ECD)单表位抗体TSP-2对酵母多糖诱发的小鼠腹膜炎的主要作用。方法用腹腔灌洗、细胞计数及特异染色、免疫组化、ELISA等各种方法检测了抗体TSP-2对模型动物的扭身次数、腹腔灌洗液的伊文思兰渗出、腹腔白细胞浸润的数量变化、腹腔肥大细胞脱颗粒情况以及腹腔灌洗液离心上清中PAF和TNFα水平的影响。结果与PBS处理组和正常兔IgG组相比,抗体TSP-2能促使小鼠因腹腔炎症引起的扭身次数减少,腹腔灌洗液中伊文思蓝OD值降低,腹腔灌洗液中白细胞渗透减少以及C48/80诱导的腹腔肥大细胞脱颗粒降低。结论抗体TSP-2可能通过对肥大细胞脱颗粒的抑制作用减轻酵母多糖诱导的小鼠腹膜炎炎症症状。Objective To observe the effect of the antibody TSP-2 against a single epitope of mouse Toll-like receptor 2 extracellular domain (mTLR2ECD) on the inflammation in mice with zymosan A-induced peritonitis. Methods In mice with peritonitis induced by intraperitoneal injection of zymosan A, pretreatments with PBS, normal rabbit IgG and TSP-2 antibody at two different doses (2.5 and 5.0 mg/kg) were administered via the tail vein. Six hours after intraperitoneal injection of zymosan A, Evans blue was injected th...

关 键 词：mTLR2 TSP-2 酵母多糖 腹膜炎 

分 类 号：R392[医药卫生—免疫学]