MAPKs信号阻断剂U0126对油酸致急性肺损伤大鼠肺泡Ⅱ型上皮细胞中AQP4表达的影响  

Effect of MAPK signal transduction pathway inhibitor U0126 on aquaporin 4 expression in alveolar type II cells in rats with oleic acid-induced acute lung injury

作　　者：陈春玲[1] 李涛平[1] 朱丽华[1] 

机构地区：[1]南方医科大学南方医院呼吸科,广东广州510515

出　　处：《南方医科大学学报》2009年第8期1525-1528,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30471720);广东省自然科学基金(04020458)

摘　　要：目的观察大鼠肺泡Ⅱ型上皮细胞(AT-Ⅱ)上AQP4与MAPKs信号转导通路在早期急性呼吸窘迫综合征时的相互联系。方法实验分正常组、油酸肺损伤组及加药组。加药组给予U0126,余两组予二甲基亚砜作为对照。测动脉血气、血管外肺水(EVLW),观察肺组织病理改变评价早期肺损伤程度。急性分离纯化大鼠AT-Ⅱ,行鞣酸染色、碱性磷酸酶染色(AKP)鉴定。采用逆转录-聚合酶链反应(RT-PCR)法检测肺泡Ⅱ型上皮细胞中AQP-4的mRNA的表达量。结果血气分析、肺组织HE染色、EVLW显示损伤组明显高于正常组,加药组较损伤组无明显变化。AQP4 mRNA表达损伤组最高,加药组较损伤组明显减少。结论大鼠肺泡II型细胞上AQP4mRNA在不同组的表达变化,表明急性肺损伤时启动了MAPKs信号传导通路且增加AQP4 mRNA的表达。Objective To investigate the relationship between aquaporin 4 (AQP4) in alveolar type II (AT-II) cells and MAPK signaling pathway in rats with early-stage oleic acid-induced acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Methods Three groups of rats, namely the normal control, ALI and U0126 treatment group were used in this study. After oleic acid-induced ALI in the latter two groups, the rats in the treatment group received 100 μmol/L U0126 treatment at the dose of 10 μl, and dimet...

关 键 词：急性肺损伤 AQP4 丝裂原活化蛋白激酶 U0126 

分 类 号：R563.8[医药卫生—呼吸系统]