蒽酮-硫酸法测定灵芝多糖的含量及其不确定度的评定  

DETERMINATION OF THE GANODERMA LUCIDUM POLYSACEHARIDE CONTENT BY ANTHRONE-SULFURIC METHOD AND EVALUATION OF UNCERTAINTY OF ANALYTICAL RESULTS

作　　者：钟岩[1] 初丽伟[1] 

机构地区：[1]吉林农业大学测试中心农业部参茸产品质量监督检验测试中心,长春130118

出　　处：《化学分析计量》2009年第5期7-10,共4页Chemical Analysis And Meterage

摘　　要：采用蒽酮-硫酸法测定了灵芝多糖的含量,并对灵芝多糖含量的不确定进行了评定。分析了测量不确定度分量的主要来源,对各不确定度分量进行了评定和计算。结果表明,灵芝多糖含量在10～60mg/mL范围内与吸光度呈良好的线性关系,线性方程为A=8.7721c+0.0187,相关系数为0.9981。当灵芝多糖含量为1.13%时,其测量结果的扩展不确定度U=0.05%(k=2)。The content of ganoderma lucidum polysaceharide was determined by the anthrone-sulfuric method,and the uncertainty of the content of ganoderma lucidum polysaceharide was evaluated.The main source of uncertainty were analyzed,and the uncertainty components were evaluated and calculated.The results showed that the linear range of ganoderma lucidum polysaceharide was 10-60 mg/mL,and the linear equation was A=8.772 1c+0.018 7,r =0.998 1.The expanded uncertainty was 0.05%(k =2)which the content of ganoderma luci...

关 键 词：灵芝多糖 蒽酮-硫酸法 不确定度 评定 

分 类 号：R284.1[医药卫生—中药学]