多重PCR方法检测猪链球菌主要致病血清型及其毒力因子  

Detection of Streptococcus suis Major Serotypes and Virulence-associated Factors by Multiplex PCR

作　　者：郑升博[1] 华修国[1] 陆伟芳[1] 袁聪俐[1] 杨志彪[1] 崔立[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2010年第1期53-58,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委重大科技攻关项目(07DZ19508);农业部公益性行业(农业)科研专项(200806016)

摘　　要：根据猪链球菌基因序列,设计合成10对引物,通过在3个反应体系Ⅰ、Ⅱ和Ⅲ中的组合优化,建立涵盖7种猪链球菌主要毒力因子mrp、epf、sly、gdh、gapdh、orf2和fbps及猪链球菌主要致病性血清型(2、7、9型)的快速敏感的多重PCR检测方法。对不同菌株检测结果显示,该多重PCR方法特异性强、敏感性高,能快速检测出猪链球菌2、7、9型及其毒力因子表型,可用于快速诊断及猪链球菌的分子流行病学调查。Ten pairs of primers were designed and synthesized according to the nucleotide sequences of Streptococcus suis.A rapid and sensitive assay,intending to detect the seven virulence-associated factors mrp,epf,sly,gdh,gapdh,orf2,fbps and major soretypes(serotype 2,7,and 9) of Streptococcus suis,was developed by optimizing and combinating the primers in three separate multiplex PCR reactions I,Ⅱ,and Ⅲ.The results for detection of various strains showed that the multiplex PCR assay was highly specific and sensiti...

关 键 词：猪链球菌 多重PCR 血清型 毒力因子 

分 类 号：S852.611[农业科学—基础兽医学]