Comparison study of trapped water in human erythrocytes by EPC and DSC method and its application  

作　　者：ZHAO Gang1, HE Liqun1, GUO Xiaojie2, LIU Zhong3, LUO Dawei1,3 & GAO Dayong1,3 1. Cryobiomedical Engineering Research Institute, Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230027, China 2. Red Cross Blood Center of Hefei, Hefei 230022, China 3. Department of Mechanical Engineering, University of Kentucky, Lexington, KY 40506-0108, USA 

出　　处：《Science China(Technological Sciences)》2004年第3期343-351,共9页中国科学（技术科学英文版）

摘　　要：The novel differential scanning calorimetry method for determining trapped water volume of human red blood cell during freezing process has been reexamined. Results show that the final erythrocyte volume is 53% of its isotonic volume after freezing to -40℃. An electronic particle counter (MultisizerTM III, Beckman Coulter Inc., USA) was used to measure cell volume changes in response to hypertonic solution. Using this approach, when extracellular solution was 3186 mOsm, the equilibrium cell volume was found to be 57% of its isotonic value. Both results indicate that 34%—40% of intracellular water is trapped and cannot respond to osmotic difference between intra- and extracellular solution. These findings are consistent with the published data: at least 20%—32% of the isotonic cell water volume is retained within RBCs during freezing. Some applications of the values of trapped water are addressed.The novel differential scanning calorimetry method for determining trapped water volume of human red blood cell during freezing process has been reexamined. Results show that the final erythrocyte volume is 53% of its isotonic volume after freezing to -40℃. An electronic particle counter (MultisizerTM III, Beckman Coulter Inc., USA) was used to measure cell volume changes in response to hypertonic solution. Using this approach, when extracellular solution was 3186 mOsm, the equilibrium cell volume was found to be 57% of its isotonic value. Both results indicate that 34%—40% of intracellular water is trapped and cannot respond to osmotic difference between intra- and extracellular solution. These findings are consistent with the published data: at least 20%—32% of the isotonic cell water volume is retained within RBCs during freezing. Some applications of the values of trapped water are addressed.

关 键 词：differential scanning calorimeter/DSC  electronic particle counter/EPC  CELL volumetric change  red blood cell/RBC/erythrocyte 

分 类 号：R319[医药卫生—基础医学]