Transfer of small chromosome fragments of Agropyron elongatum to wheat chromosome via asymmetric somatic hybridization  

作　　者：WANG Jing,XIANG Fengning,XIA Guangmin & CHEN Huimin School of Life Sciences,Shandong University,Jinan 250100,China 

出　　处：《Science China(Life Sciences)》2004年第5期434-441,共8页中国科学（生命科学英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grant No.30370857);Major Project of Ministry of Education in China;National 863 High Technology Research and Development Project(No.2001AA241032).

摘　　要：The chromosome constitution of hybrids and chromation patterns of Agropyro-nelelongatum(Host)Neviski in F5 somatic hybrid lines Ⅱ -1-3 and l-1-9 between Triticum aesti-vum L.and A.elongatum were analyzed.Based on the statistic data of pollen omther cells,F5 l-1-9 and Ⅱ-1-3 had 20—21 bivalents with a frequency of 84.66% and 85.28%,of which,89.83% and 89.57% were ring bivalents.The result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior.RAPD analysis showed that the two hybrids contained biparental and integrated DNA.GISH(Genome in situ hybridization)revealed that in the form of small chromosome segments,A.elongatum chromatin was scattered on 4—6 wheat chromo-somes near by the region of centromere and telomere in the two hybrid lines.SSR analysis in-dicated that A.elongatum DAN segments were distributed on the 2A,5B,6B and 2D wheat chromosomes in the hybrids,which was in accordance with the GISH results that small-segments intercalated poly-site.The chromosome constitution of hybrids and chromation patterns of Agropyro-nelelongatum(Host)Neviski in F5 somatic hybrid lines Ⅱ -1-3 and l-1-9 between Triticum aesti-vum L.and A.elongatum were analyzed.Based on the statistic data of pollen omther cells,F5 l-1-9 and Ⅱ-1-3 had 20—21 bivalents with a frequency of 84.66% and 85.28%,of which,89.83% and 89.57% were ring bivalents.The result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior.RAPD analysis showed that the two hybrids contained biparental and integrated DNA.GISH(Genome in situ hybridization)revealed that in the form of small chromosome segments,A.elongatum chromatin was scattered on 4—6 wheat chromo-somes near by the region of centromere and telomere in the two hybrid lines.SSR analysis in-dicated that A.elongatum DAN segments were distributed on the 2A,5B,6B and 2D wheat chromosomes in the hybrids,which was in accordance with the GISH results that small-segments intercalated poly-site.

关 键 词：TRITICUM AESTIVUM L. Agropyron elongatum(Host)Neviski F5 SOMATIC hybrid lines small CHROMOSOME segment translocation in situ hybridization simple sequence repeat. 

分 类 号：S512.1[农业科学—作物学]