脐带间充质干细胞体外分化为有功能的低免疫原性肝细胞样细胞  

Differentiation of Human Umbilical Cord Derived Mesenchymal Stem Cells into Low Immunogenic and Functional Hepatocyte-like Cells in vitro

作　　者：任红英[1] 赵钦军[1] 邢文[1] 杨少光[1] 卢士红[1] 任倩[1] 张磊[1] 韩忠朝[1] 

机构地区：[1]中国医学科学院北京协和医学院血液学研究所实验血液学国家重点实验室,天津300020

出　　处：《中国医学科学院学报》2010年第2期190-194,246,共6页Acta Academiae Medicinae Sinicae

基　　金：国家高技术研究发展计划项目(863项目) (2006AA02A110)~~

摘　　要：目的观察人脐带来源的间充质干细胞(UC-MSCs)体外诱导分化为肝细胞样细胞(DHC)后,是否具有肝细胞的生物学特性和功能,以及其免疫原性的变化。方法采用改良的二步法肝细胞分化培养体系体外诱导UC-MSCs向肝细胞分化,并用RT-PCR和免疫荧光染色法检测诱导后不同时间DHC肝细胞特异性标记的表达;采用ELISA法检测细胞培养液中白蛋白(ALB)和尿素的浓度,以及混合淋巴细胞培养检测DHC对淋巴细胞增殖能力的影响。结果 UC-MSCs低表达甲胎蛋白(AFP)、ALB和细胞角蛋白-19(CK-19)的mRNA和蛋白,成肝诱导分化后14和28dDHC均高表达肝细胞标志AFP、ALB、CK-19以及色氨酸2,3-加双氧酶基因。诱导后的DHC能够以时间依赖方式产生ALB和分泌尿素,不表达人白细胞DR抗原,而且能够抑制淋巴细胞增殖。结论 UC-MSCs在体外能够分化为有功能的DHC且具有低免疫原性的特征。Objective To investigate the biological function of hepatocyte-like cells derived from mesenchymal stem cells that isolated from human umbilical cord (UC-MSCs)in vitro,and to detect the changes in the immunogenicity of the differentiated hepatocyte-like cells (DHC).Methods Transdifferentiation of UC-MSCs into hepatic lineage in vitro was induced in modified two-step induction medium.The expressions of hepatic specific markers were detected by RT-PCR analysis and immunofluorescence staining at different time...

关 键 词：间充质干细胞 肝细胞样细胞 分化 免疫原性 

分 类 号：Q25[生物学—细胞生物学]