In vitro Study on Human Trophoblast Cells Infected with HCMV  

作　　者：肖娟 张丹丹 陈娟娟 尹宗智 刘涛 艾继辉 陈素华 

机构地区：[1]Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2010年第1期94-97,共4页华中科技大学学报（医学英德文版）

基　　金：supported by grants from National Natural Sciences Foundation of China(No.30672243,30671093)

摘　　要：Human trophoblast cells were isolated and cultured in vitro in order to investigate possible pathogenesis of intrauterine infection caused by HCMV.Trophoblast cells were obtained by compound enzymes digestion and discontinuous percoll gradient.Cells and purity were identified by using immunocytochemistry assay with anti-CK7,Vim and β-hCG antibodies.HCMV AD169 strain replication in isolated trophoblast cells and cell apoptosis were detected at different time points post infection(p.i.).The results showed tha...Human trophoblast cells were isolated and cultured in vitro in order to investigate possible pathogenesis of intrauterine infection caused by HCMV.Trophoblast cells were obtained by compound enzymes digestion and discontinuous percoll gradient.Cells and purity were identified by using immunocytochemistry assay with anti-CK7,Vim and β-hCG antibodies.HCMV AD169 strain replication in isolated trophoblast cells and cell apoptosis were detected at different time points post infection(p.i.).The results showed tha...

关 键 词：CYTOMEGALOVIRUS trophoblast cells isolation and purification biological characteristics 

分 类 号：R714.2[医药卫生—妇产科学]