Construction and Identification of a Yeast Two-Hybrid Bait Vector and Its Effect on the Growth of Yeast Cells and the Self-Activating Function of Reporter Genes for Screening of HPV18 E6-Interacting Protein  

作　　者：梅泉 李双 刘萍 奚玲 王世宣 孟玉菡 刘杰 杨欣慰 卢运萍 汪辉 

机构地区：[1]Cancer Biology Research Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology [2]Department of Obstetrics and Gynecology,The Second People's Hospital of Yichang City [3]Family Planning Research Institute,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2010年第1期8-12,共5页华中科技大学学报（医学英德文版）

基　　金：supported by grants from National Natural Sciences Foundation of China(No.30672227,30600668);"973"Program of China(No.2009CB521800);Joint Research Fund for Overseas Chinese,Hong Kong and Macao Young Scholars(No.30628029)

摘　　要：By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...

关 键 词：HPV18 E6 yeast two-hybrid system GENE bait plasmid 

分 类 号：R341[医药卫生—基础医学]