荷移荧光光谱法测定阿米卡星  

Fluorescence spectrometric determination of amikacin by charge transfer reaction

作　　者：陈智娴 戈延茹[2] 

机构地区：[1]江苏省南通卫生高等职业技术学院,南通226007 [2]江苏大学药学院,镇江212013

出　　处：《分析试验室》2010年第5期114-116,共3页Chinese Journal of Analysis Laboratory

摘　　要：建立了测定痕量阿米卡星制剂的新方法。研究了阿米卡星(AK)与电子受体2,3-二氰-5,6-二氯-1,4-对苯醌(DDQ)之间的荷移反应。在甲醇中,阿米卡星与DDQ在室温(30℃)下可迅速形成荷移络合物,其荧光强度较阿米卡星显著增强,最大激发和发射波长分别红移38 nm和86 nm。阿米卡星含量在0.1~1.8×10-6mol/L范围内与其荧光强度呈良好的线性关系,相关系数r为0.9992,检出限为0.0244 mg/L。本法用于实际样品分析,回收率为96.1%~103%,相对标准偏差(RSD)为1.2%~1.7%。A new sensitive method for rapidly determination of the content of amikacin in preparations is established.The charge transfer complex formed between amikacin as the donor and DDQ as the accepter has been studied by fluorimetry.Amikacin reacted with DDQ in absolute methanol at 30 ℃ to form the complex.Its fluorimetric intensity was stronger than that of amikacin,and the excitation and emission peaks of the complex were red shifted by 38 nm and 86 nm,respectively.A linear calibration graph was obtained over ...

关 键 词：阿米卡星 2 3-二氰-5 6-二-氯1 4-对苯醌 荧光光谱 荷移反应 

分 类 号：R927.2[医药卫生—药学]