基于聚硫堇和纳米银固定酶的葡萄糖生物传感器  

A glucose biosensor based on a poly(thionine) and Ag colloids modified glassy carbon electrode

作　　者：李群芳[1] 郑丽敏[1] 娄方明[1] 张倩茹[1] 丁雁南[1] 

机构地区：[1]遵义医学院药学院,遵义563003

出　　处：《分析试验室》2010年第5期102-105,共4页Chinese Journal of Analysis Laboratory

基　　金：遵义医学院硕士科研启动基金(2008F-305)项目资助

摘　　要：用循环伏安法将电子媒介体硫堇电聚合在玻碳电极表面上,使其表面形成均匀的带负电的聚硫堇膜,通过静电吸附作用吸附表面带正电荷的纳米银溶胶,接着通过静电吸附带负电的葡萄糖氧化酶,最后用聚硫堇包埋电极,从而制得性能优良的葡萄糖氧化酶(GOD)生物传感器。实验发现传感器氧化峰电流与葡萄糖的浓度在1.0×10-8~5.0×10-6mol/L(r=0.9963)范围内呈良好线性关系,检出限为5.0×10-9mol/L(S/N=3)。A novel method for the preparation of the glucose biosensor was described.Thionine was electropolymerized on the glassy carbon electrode to form a negatively charged surface by cyclic voltammetry.Nano-Au/glucose oxindase(GOX) were immobilized on the electrode modified by poly(thionine) via electrostatic adsorption respectively,then the modified electrode was combined with poly-thionine to prepare the glucose biosensor.The biosensor shows fast response,high sensitivity and stability.The linear response of th...

关 键 词：聚硫堇 葡萄糖氧化酶 纳米银 葡萄糖生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]