中空纤维协同液相微萃取在4种莨菪碱分析中的应用  

Application of hollow fiber cooperative liquid phase microextraction in analysis of four hyoscyamines

作　　者：席国臣[1] 陈璇[1] 白小红[1] 黄菲[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《分析试验室》2010年第4期1-5,共5页Chinese Journal of Analysis Laboratory

基　　金：山西省自然科学基金(2007011086);太原市大学生创新创业基金(08122034)项目资助

摘　　要：利用协同液相微萃取方法初步探讨氢溴酸山莨菪碱、硫酸阿托品、氢溴酸东莨菪碱和丁溴酸东莨菪碱的优势构象;阐明有机溶剂对4种莨菪碱的萃取选择性和协同萃取作用机理;探讨中空纤维与莨菪碱形成电荷转移超分子对萃取效果的影响以及4种莨菪碱结构中N原子的亲电能力;结合高效液相色谱技术测定药物制剂中4种莨菪碱的含量。4种莨菪碱的线性范围均为0.05～5mg/L(r>0.99),方法RSD小于7%,消旋山莨菪碱片和颠茄片中平均回收率分别为95.0%～119.0%和93.0%～95.0%,氢溴酸山莨菪碱和丁溴酸东莨菪碱检出限为0.03mg/L,硫酸阿托品和氢溴酸东莨菪碱检测出限为0.01mg/L。The preferred conformations of anisodamine hydrobromide,atropine sulphate,scopolamine hydrobromide,scopolamine butylbromide were researched by cooperative liquid phase microextraction (CLPME) initially.In addition,the selective extraction and cooperative extraction mechanism of organic solvent to the standards were interpreted.The effect of charge transfer supermolecule formed by hollow fiber and hyoscyamines on the extraction efficiency was researched in the extraction procedure,and the electrophilic capab...

关 键 词：协同液相微萃取 优势构象 电荷转移超分子 莨菪碱 

分 类 号：R284[医药卫生—中药学]