黑暗诱导烟草单个叶片衰老的研究  

In vivo study in dark-induced senescence in individual tobacco leaf

作　　者：姚珊珊[1] 朱晓宇[1] 赵二卫[1] 赵宸楠[1] 崔红[1] 

机构地区：[1]河南农业大学烟草学院烟草行业栽培重点实验室,郑州市文化路95号450002

出　　处：《中国烟草学报》2010年第2期36-39,共4页Acta Tabacaria Sinica

基　　金：烟草行业栽培重点实验室科技项目(06TCKL06004)

摘　　要：采用铝箔纸包裹的方法,对生长中的烟草单个叶片进行黑暗处理(DIS)。发现在处理第5d叶片明显变黄,叶片衰老标记基因CP1表达量开始明显增高;与黑暗诱导的离体叶片相比,黑暗诱导的生长叶片在叶绿素、可溶性蛋白、MDA含量以及SOD、POD酶活等指标上,呈现相似但较为平缓的变化趋势。该结果可为进行烟草叶片衰老研究提供理想的实验体系。Individual tobacco leaf was kept in dark environment in vivo by aluminum foil paper to induce senescence and to study the process of quality formation.RNAs of senescence-specific cysteine protease(CP1)were detected wiht RT-PCR on 3rd day after treatment,and leaf became yellowish on 5th day.A comparative analysis of darken individual leaf in vivo(DIS)and dark-incubated leaf in vitro(DET)revealed similar changes in chlorophyll,soluble protein content and SOD,POD activity during darkening.Both senescence proce...

关 键 词：烟草 黑暗 衰老 单个叶片 

分 类 号：S572.01[农业科学—烟草工业]