小麦多子房性状近等基因系遗传背景的分子标记检测  

Molecular Marker Detection of Genetic Background in Near-isogenic Lines with Multi-ovary Character

作　　者：朱东旭[1] 马守才[1] 张改生[1] 牛娜[1] 

机构地区：[1]西北农林科技大学陕西省作物杂种优势研究与利用重点实验室/小麦育种教育部工程研究中心,陕西杨凌712100

出　　处：《麦类作物学报》2010年第3期406-410,共5页Journal of Triticeae Crops

基　　金：国家高技术研究发展计划(863计划)重大专项基金(2009AA101102);西北农林科技大学唐仲英育种基金

摘　　要：小麦多子房性状具有明显的穗粒数优势,可明显提高小麦的繁殖系数。为研究多子房这一特殊性状,以矮败小麦为杂交工具材料、多子房小麦多Ⅱ为供体亲本、单子房小麦77(2)为轮回亲本,经过连续回交5代和自交,初步培育成多子房近等基因系(NIL)。在小麦21对染色体的各长短臂随机选取了42对SSR引物,对23个NIL材料及其轮回亲本的遗传相似性和遗传距离进行了比较分析,结果表明:(1)42对SSR引物共扩增出92条谱带,其中53条具有多态性,多态性条带率57.61%;30对引物具有多态性,多态性引物率71.43%;(2)23个材料与轮回亲本的遗传相似系数为0.77~0.95,平均遗传相似系数为0.88;(3)聚类分析将23个材料和轮回亲本的相似系数在0.84处分为7大类,其中70.83%的材料归入第一类;第一类在0.87处又分为3个亚类,其中多子房材料2008H69与轮回亲本差异最小,在0.93处聚为一类。Multi-ovary character as a new peculiar properties of wheat had an advantage of kernels per head obviously,and could improve the propagation coefficiently.In this study,dwarfing sterile wheat was used as a tool material,duo II was used as donor parent,and 77(2)was used as recurrent male parent.The multi-ovary near-isogenic lines(NIL) was preliminary produced after successive backcrossing(5 times) and crossing.42 pairs of SSR primers were selected randomly which located in short or long arm of 21 wheat chrom...

关 键 词：多子房小麦 近等基因系 SSR分子标记 

分 类 号：S512.1[农业科学—作物学]