农杆菌浸花对小麦结实及种子萌发和幼苗生长发育的影响  

Influence of Floral Dipping with Agrobacterium on Wheat Seed Setting and Seeds Germination

作　　者：杨进军[1] 宋立立[1] 白娜[1] 徐开杰[1] 刘曙东[1] 奚亚军[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《麦类作物学报》2010年第3期529-534,共6页Journal of Triticeae Crops

基　　金：转基因生物新品种培育科技重大专项(2008ZX08002-003);西北农林科技大学青年学术骨干支持计划(01140306)

摘　　要：为了明确农杆菌浸花处理对小麦结实及后代种子萌发的影响,用小麦品种郑麦9023、西农889和西农979进行了不同浓度农杆菌菌液的浸花处理。结果表明,小麦基因型和农杆菌菌液浓度对小麦结实率、千粒重、种子发芽率、幼苗高度、幼苗鲜重、幼苗干重、幼苗根长、叶绿素含量、MDA含量、POD、SOD活性及卡那霉素抗性苗率影响的互作效应不显著;随着农杆菌菌液浓度的增加,不同小麦品种的结实率、种子发芽率、幼苗高度、幼苗鲜重、幼苗干重、幼苗根长、叶绿素含量、POD及SOD活性呈降低趋势;MDA含量和卡那霉素抗性苗率总体随着农杆菌菌液浓度的增加而增加;小麦品种对农杆菌的反应存在一定基因型差异。综合分析农杆菌对小麦结实和种子萌发的影响及卡那霉素抗性苗率,农杆菌浸花处理的使用浓度在1.0~1.5OD范围可能较为适宜。With the wheat varieties of Zheng 9023,Xinong889,Xinong979 as the dominated factors,the agrobacterium density of 0,0.5,1.5 and 2.5 OD as the deputy factors,the effect on wheat seed-setting and germination of seeds of agrobacterium treatment on spikelet were studied by resplit-plot design.The results showed that the interaction effects of varieties and agrobacterium density on the seed-setting rate,thousand-grain weight,seed germination rate,seedling height,seedling fresh weight,seedling dry weight,seedling ...

关 键 词：小麦 农杆菌 浸花 结实 种子萌发 

分 类 号：S512.1[农业科学—作物学]